摘要
目的 探讨X射线修复交叉互补基因1 （XRCC1）基因多态性和低剂量电离辐射暴露对放射工作人员外周血淋巴细胞微核的影响.方法 采用病例对照研究设计,将2013至2015年成都市1 102例放射工作人员中检出微核的45例放射诊断人员作为病例组,按性别、年龄（≤40岁或＞40岁）1∶4配对,选择未检出微核的180例放射诊断人员作为对照组,按检出微核的水平（0‰、1％、2％）将研究对象分为对照组（180人）、低检出组（34人）和中检出组（11人）;采用《放射工作人员职业健康检查表》收集研究对象的一般基线情况、吸烟饮酒史、有毒有害物质接触史、既往疾病史、放射暴露累积剂量和淋巴细胞微核率（％）等;采用限制性片段长度多态性聚合酶链反应（PCR-RFLP）技术进行基因分型,比较不同微核检出组间基线情况及放射暴露水平差异,采用多分类logistic回归分析XRCC1 Arg399Gln基因多态性和累积剂量的组合效应对微核发生的影响.结果 对照组、低检出组和中检出组放射诊断人员放射暴露累积剂量为（23.44±15.23）、（21.76±2.56）、（24.22± 18.61） mSv,组间差异无统计学意义（P＞0.05）;调整年龄、吸烟、饮酒因素后,多分类logistic回归分析结果显示,在显性遗传模式下,以携带Arg399Gln-GG基因型为对照,中检出组携带Arg399Gln-GA ＋AA基因型能够降低微核发生的风险（OR =0.175,95％ CI：0.036～0.848）;Arg194Trp、Arg280His两个位点对微核发生影响无统计学意义（P＞0.05）,XRCC1 Arg399Gln基因多态性和累积剂量的组合效应对微核发生的影响无统计学意义（P＞0.05）.结论 XRCC1 Arg399Gln位点基因多态性可影响微核的发生,且携带XRCC1 Arg399Gln-GA＋AA基因型是微核发生的保护因素,低剂量电离辐射可能不独立影响微核的发生.
Objective To explore the effects of X-ray repair cross complementing gene 1 （XRCC1） polymorphism and low dose ionizing radiation exposure on radiology professionals＇ peripheral blood lymphocyte micronucleus.Methods A matched case-control study was designed.From 2013 to 2015,1 102 radiology professionals with micronucleus test rusults,and 45 cases with present micronucleus were enroled into case group.180 diagnostic radiology technicians detecting no micmnucleus were chosen as control group,cases and controls were 1：4 mached on gender,age ≤40 or 〉40 years old.According to the detection of micronucleus levels （0%,1%,2%）,the objects of our study were divided into the reference group,the low detection group and the medium detection group.The form of radiation workers＇ occupational health examination was used to collect the general baseline of the research objects,history of smoking,drinking,poisonous and harmful material exposure,past medical history,accumulated illuminated dose and lymphocyte micronucleus rates （%）,etc.Using restriction fragment length polymorphism polymerase chain reaction （RFLP-PCR） technology for genotyping;Compared the baseline data and radiation exposure level between the differentmicrokernel detection groups;Adopted multivariate logistic regression to analysis the combination effect of XRCC1 Arg399Gln gene polymorphism and accumulated illuminated dosefor micronucleus rate.Results The accumulated illuminated dose in the reference group,the low detection group and the medium detection group were （23.44±15.23）,（21.76±2.56）,（24.22±18.61） mSv,respectively.There was no statistically significant difference among the groups （P〉0.05）.Under the dominant inheritance mode,after adjusted age,smoking and drinking factors,the results suggested that XRCC1 Arg399Gln micronucleus medium detection group compared with the reference group,Arg399Gln-GG as reference,Arg399Gln-GA＋AA decreased the occurrence of micronucleus （OR =0.175,95% CI：0.036-0.848）.Arg194Trp
出处
《中华劳动卫生职业病杂志》
CAS
CSCD
2017年第3期189-192,共4页
Chinese Journal of Industrial Hygiene and Occupational Diseases
基金
基金项目：成都市科技惠民项目资助（2014-HM01-00396-SF）
关键词
微核
X射线修复交叉互补基因1
基因多态性
辐射
电离
Micronucleus
X-ray repair cross complementing gene 1
Gene polymorphism
Radiation,ionizing
