摘要
利用柱后分流频率调制Chirp Z变换离子迁移谱(IMS),建立了高效液相色谱与纳流电喷雾阵列Chirp Z变换离子迁移谱(HPLC-Nano ESI-Chirp Z IMS)联用分析方法,考察了喷雾电压、溶剂组成、溶液流速等参数对Nano ESI-Chirp Z IMS信噪比的影响。在此基础上,利用建立的方法对一系列四烷基溴化铵类化合物进行测定,并比较了Chirp Z变换法和FT变换法两种方法的信噪比和分辨率。实验结果表明,最佳实验条件为:喷雾电压4.5 k V;溶剂组成90%甲醇;ESI溶液流速为8μL/min。利用HPLC-Nano ESI-Chirp Z IMS联用方法成功测定了四丁基溴化铵、四戊基溴化铵、四己基溴化铵、四庚基溴化铵、四辛基溴化铵烷、四癸基溴化铵组成的混合样品。与传统信号平均离子迁移谱相比,Chirp Z变换离子迁移谱的信噪比更高,其迁移时间的测定精度优于傅里叶变换离子迁移谱。
By combining frequency modulation Chirp Z transform ion mobility spectrometers （IMS） and multi nozzle electrospray array ionization source, a method of NanoESI-Chirp Z transform ion mobility spectrometry- high performance liquid chromatography was developed for the determination of n-alkyl ammonium bromide compounds. The parameters of NanoESI-Chirp Z transform IMS such as electric field intensity, solvent composition, and solution flow rate were investigated and optimized. Subsequently, four kinds of n-alkyl ammonium bromide compounds were respectively detected by this developed Chirp Z transform method and Fourier transform method, and the obtained results were compared. The result indicated that the optimum conditions were electric field intensity of 4. 5 kV, and ESI solution flow rate of 8μL/min. Then a test mixture containing tetrabutylammonium bromide, tetrapentylammoniumbromide, tetrahexylammonium bromide, tetraheptylammonium bromide, tetranoctylammoniumbromideandtetrakis （ decyl ） ammonium bromide was successfully separated and determined by the HPLC- nanoESI-Chirp Z IMS method. Chirp Z transform method provided higher signal to noise ratio compared to conventional signal averaging method, and was superior to FT method in the determination of drift time.
出处
《分析化学》
SCIE
EI
CAS
CSCD
北大核心
2017年第6期809-816,共8页
Chinese Journal of Analytical Chemistry
基金
兵团国际合作项目(No.2015AH005)
研究生创生项目(No.TDGRI 201523)
国家自然科学基金项目(No.21365018)资助~~
关键词
CHIRP
Z变换
离子迁移谱
高效液相色谱
Chirp Z transform
Ion mobility spectrometry
High performance liquid chromatography
