摘要
紫苏是我国卫生部首批公布的60种药食两用型植物之一,用途广泛。目前随着紫苏产业的发展,优良品种的选育与应用推广已成为限制其发展的主要瓶颈。该研究报道通过系统选育,结合分子标记辅助鉴定的方法进行紫苏新品种选育。通过全基因组测序,根据已有的基因集对检测到的变异进行注释,并与紫苏常见变异数据库比对分析,最后筛选出30个非同义突变SNPs标记作为中研肥苏1号特征性SNP标记,用于紫苏新品种的材料鉴选。Z最终选育形成具有叶籽两用,丰产,高抗,耐瘠等特性,可做绿肥使用的中研肥苏1号紫苏新品种。中研肥苏1号通过北京市植物新品种鉴定,鉴定编号为京品鉴药2016054。分子标记辅助鉴定指导新品种选育,可为药用植物育种提供新的参考。
Perilla frutescens is one of 60 kinds of food and medicine plants in the initial directory announced by health ministry of China. With the development of Perilla domain in recent,the breeding and application of good varieties has become the main bottleneck of its development. This study reported that applied to the system selection,add to marker-assisted method to breed perilla varieties. Through the whole genome sequencing and consistency matching,annotated the mutation locus according to genome data,and comparison analysis with Perilla common variants database,finally selected 30 non-synonymous mutation SNPs used as characteristic markers of Zhongyan Feishu No. 1. those SNP marker were used as chosen standard of Perilla varieties. Finally breeding new perilla variety Zhongyan Feishu No. 1,which possess to characters of the leaf and seed dual-used,high yield,high resistance,and could used to green fertilizer. The Zhongyan Feishu No. 1 acquired the plant new varieties identification of Beijing city,the identification numbers is 2016054. Marker assisted identification guide new varieties breeding in plants,which can provide a new reference for breeding of medicinal plants.
出处
《中国中药杂志》
CAS
CSCD
北大核心
2017年第9期1668-1672,共5页
China Journal of Chinese Materia Medica
基金
国家自然科学基金项目(31360067)
关键词
紫苏
品种选育
SNP
分子辅助鉴定育种
Perilla frutescens
variety breeding
NP
marker assisted identification breeding
