摘要
目的采用HPLC测定大鼠体内外消化道组织液中芦丁含量方法,研究芦丁在大鼠体内和体外的分布转化规律。方法将大鼠分为活体和离体两组,活体组灌胃给芦丁提取液,离体组摘取组织后模拟体内环境离体给药;然后分别提取组织液,采用HPLC测定芦丁在大鼠胃、大肠、小肠中的含量。结果芦丁在大鼠体内的平均分布含量:小肠>大肠>胃,芦丁在大鼠体外的平均分布含量:胃>大肠>小肠,体内体外相互比较,芦丁的平均分布含量在小肠中差异最大。结论大鼠的胃、大肠、小肠对芦丁都有吸收,其中芦丁的平均分布含量在小肠中差异最大,小肠可能为芦丁摄取和转化的主要部位。
Aim To determinate the rutin content from gastrointestinal tissues of rats in vivo and in vitro by HPLC, and study the regularity of distribution and transformation of rutin in vivo and in vitru. Methods Rats were divided into two groups, in vivo and in vitro. The group in vivo were filled the stomach to extract rutin, another group were delivered drug by simulating the environment in vivo after gather the tissues. Then extract tissue fluid and determinate the rutin content in stomach, large intestine and small intestine by HPLC. Results The average distribution of rutin content in rats in vivo： small intestine 〉large intestine 〉stomach, and the average distribution of rutin content in vitro： stomach〉large intestine〉small intestine. To compare the in vitro and in vitro, differences in the average distribution of rutin content in the small intestine is the largest. Conclusion The rat stomach, large intestine, small intestine have absorbed on rutin. Among them, differences in the average distribution of rutin content in the small intestine is the largest. Srnall intestine could be main parts of the absorption and transformation of rutin.
出处
《广东化工》
CAS
2017年第9期64-66,共3页
Guangdong Chemical Industry
基金
辽宁省大学生创新创业训练项目(项目编号:201510162000035)
