摘要
采用气相色谱-串联质谱法快速测定鸡肉组织中的氯羟吡啶残留,样品经乙腈-水提取,冷冻脱脂后,直接以丙酸酐衍生,采用气相色谱-串联质谱(GC-MS/MS)进行分析,多反应监测(MRM)模式检测。结果表明,氯羟吡啶在0.5~40 ng/m L浓度范围内呈良好的线性关系,方法的定量下限为5.0μg/kg,回收率为101.5%~111.8%,相对标准偏差(RSD)小于7.7%。该方法简便、快速、灵敏度高,重现性好,可用于鸡肉组织中氯羟吡啶的快速确证检测。
A rapid method was established for the determination of clopidol in chicken by gas chromatography - tandem mass spectrometry（ GC - MS/MS）. Samples were extracted with acetonitrile - water. The extract was frozen to remove the lipid within, and derivated with propionic anhydride. Then, it was analyzed by GC - MS/MS under MRM mode. The calibration curve for clopidol was linear in the concentration range of 0. 5 - 40 ng/mL with a correlation coefficient of 0. 999 4. The limit of quantitation for this method was 5.0 μg/kg. The average recoveries at spiked concentrations of 5.0, 10, 20μg/kg ranged from 101.5% to 111.8% , with relative standard deviations less than 7.7% . The method is simple, quick and high sensitive, and could be applied to detect clopidol residues in real samples.
出处
《分析测试学报》
CAS
CSCD
北大核心
2017年第5期689-692,共4页
Journal of Instrumental Analysis
基金
国家质检总局科研计划项目(2016IK169)
关键词
氯羟吡啶
气相色谱-串联质谱
鸡肉
clopidol
gas chromatography- tandem mass spectrometry（ GC -MS/MS）
chicken
