摘要
目的通过基因表达汇编(GEO)公共数据集研究载脂蛋白D(ApoD)在不同分期及不同类型胃癌患者中的表达情况,探讨ApoD与胃癌发生及发展的关系,为临床诊疗及风险预测提供依据。方法从美国国家生物技术信息中心(NCBI)的GEO数据库(http://www.ncbi.nlm.nih.gov/geo)中下载基因表达谱公共数据集GSE62254,纳入标准为包含ApoD表达数据的胃癌肿瘤表达谱。探究ApoD表达与胃癌临床病理学参数之间的相关性及其对预后的影响。采用基因集富集分析(GSEA)法预测ApoD的调控基因。结果 ApoD在女性、年龄≤60岁及弥漫型胃癌的患者中高表达,同时其表达与胃癌的T分期、临床分期呈正相关。在预后方面,ApoD高表达的胃癌患者无进展存活时间、总存活期明显短于低表达患者(P<0.05)。ApoD高表达样本富集了细胞黏附、紧密连接、花生四烯酸代谢等基因集。结论在胃癌中,ApoD高表达为预后不良因素,可作为影响胃癌患者预后的判断因素。
Objective To explore apolipoprotein D（ApoD） expression and its clinical value in gastric cancer,and to predict the effect of ApoD in development of gastric cancer. Methods Get the datasets of gastric cancer GSE62254 from Gene Expression Omnibus （GEO） database from NCBI（ http ：//www. ncbi. nlm. nih. gov/geo）. Inclusion criteria was to include data of stomach tumor of ApoD expression spectrum. The correlation between ApoD expression and clinical pathological characteristics were analyzed. The regulatory gene of APOD was predicted by Gene Set Enrichment Analysis （GSEA）. Results More high expression of ApoD was found in famale and patients age less than 60 ; expression was higher in the diffuse gastric cancer, and the expression of ApoD was positively correlated with T stage,TNM stage. In prognosis aspect, ApoD high expression in patients with gastric cancer, disease-free survival and overall survival was much shorter than that in patients with lower expression（P 〈0. 05）. The related gene atlases on cell adhesion,tight junction,arachidonic acid metabolism were rich in the high expression samples of ApoD. Conclusion The expression of ApoD indicates poor prognosis of gastric cancer and can be used as a potential prognostic marker.
出处
《临床军医杂志》
CAS
2017年第4期387-389,393,共4页
Clinical Journal of Medical Officers
基金
辽宁省科技厅计划项目(2014225013)
辽宁省科技厅计划项目(2014226033)
中医药临床学(专)科能力建设项目
关键词
载脂蛋白D
胃癌
癌基因组图谱
预后
Apolipoprotein D
Gastric cancer
The cancer genome atlas
Prognosis
