摘要
鼠李糖脂是最常见,研究最深入,应用最广泛的一类生物表面活性剂。从油田附近、沼气池旁的土壤中分离得到了25株菌,通过硫酸-苯酚反应,乳化实验,排油性实验,薄层色谱实验筛选产鼠李糖脂的菌株并表征产生的鼠李糖脂,通过16S r DNA序列确定细菌的种属。硫酸-苯酚反应显示有2株菌可能产鼠李糖脂;5株菌的发酵液具有明显的乳化效果,命名为"其红"这株菌的乳化指数可达58.97%;1株菌的发酵液上清稀释10倍后排油圈直径仍可达3.53 cm;薄层色谱实验也显示"其红"菌产鼠李糖脂。在四个实验中"其红"菌都检测到了鼠李糖脂,故确认"其红"菌可以产鼠李糖脂。16S r DNA序列分析表明"其红"菌属于希瓦氏菌属(Shewanella)并与腐败希瓦菌(S.putrefaciens LMG 26268(T))相似度最高。
Rhamnolipid is the most common biosurfactant that has been deepest studied and the most widely applied. Total 25 strainswere isolated from soil of the near oil field and biogas plant. The strains that produced rhamnolipid were screened and characterized by sulfuricacid-phenol reaction,emulsification experiment,oil-spreading experiment,and thin layer chromatography experiment. The strains wereidentified by 16 S r DNA sequence analysis. The sulfuric acid-phenol reaction showed that 2 strains might produce rhamnolipid;the fermentationsupernatants of 5 strains showed emulsified effect and the emulsifying index of the strain was up to 58.97%. The oil-spreading diameter of thefermentation supernatant,diluted 10 times,of one strain reached 3.53 cm. The thin layer chromatography experiment verified that the strain＂Qihong＂ did produce rhamnolipid. The rhamnolipid in the strain ＂Qihong＂ was detected by all four experiments,confirming that strain ＂Qihong＂produced rhamnolipid. The 16 S r DNA sequence analysis revealed that the strain ＂Qihong＂ belonged to Shewanella,and was the most similar toShewanella putrefaciens LMG 26268（T）.
作者
王江
鞠酒
曹海龙
郭卫华
尹恒
WANG Jiang JU Jiu CAO Hai-long GUO Wei-hua YIN Heng(College of Fisheries and Life Science, Dalian Ocean University, Dalian 116023 Natural Products and Glyco-Biotechnology, Dalian Institute of Chemical Physics, Chinese Academy of Sciences, Dalian 116023 College of Food Science and Engineering, Dalian Ocean University, Dalian 116023)
出处
《生物技术通报》
CAS
CSCD
北大核心
2017年第4期191-197,共7页
Biotechnology Bulletin
基金
中科院青年创新促进会(2015144)
大连市杰出青年科技人才(2015R010)
