摘要
肉桂醛改性处理的壳聚糖对碳纳米管表面进行修饰,制得一种新型的肉桂醛/壳聚糖/碳纳米管复合材料。用傅立叶红外光谱(FT-IR)、X射线衍射仪(XRD)、热分析系统(TGA)、透射电镜(TEM)对该肉桂醛/壳聚糖/碳纳米管复合材料及相应中间产物进行表征与分析。以猪胰脂肪酶为模型,探究该肉桂醛/壳聚糖/碳纳米管复合材料固定化酶的性质。结果表明:在肉桂醛与壳聚糖中游离氨基的物质的量之比为4:1、pH 7.0及脂肪酶浓度为5 mg/mL时,该复合材料最大负载量为248 mg/g,酶活性为8 064U/g,重复利用7次后,酶活性仍然保持69%。
Using chitosan modified by cinnamaldehyde to decorate carbon nanotubes,a novel cinnamaldehyde/chitosan/carbon nanotubes composites were synthesized.The modified composites and its corresponding intermediates were characterized by FT-IR,XRD,high-resolution SEM and TGA.Taking porcine pancreatic lipase as a model,explored the properties of cinnamaldehyde/chitosan/carbon nanotubes composite materials.The results indicated that when the conditions were：molar ratio of cinnamaldehyde to chitosan of 4：1,pH =7.0and the enzyme concentration of 5mg/mL,and the maximum load of 248mg/g and the enzyme activity of 8 064U/g were obtained.The enzyme activity was still above 69%after seven recycles.
出处
《食品与机械》
CSCD
北大核心
2017年第3期8-12,70,共6页
Food and Machinery
基金
江苏省自然科学基金(编号:BK20161133)
关键词
碳纳米管
肉桂醛
壳聚糖
非共价修饰
固定化酶
carbon nanotubes
cinnamaldehyde
chitosan
non covalent modification
immobilization of enzyme
