摘要
目的探讨癌基因K-ras及C-myc在宫颈癌中的表达及临床意义。方法选择2010年12月-2016年6月在该院因TCT结果异常或HPV-DNA检测异常行阴道镜宫颈活检术的389例患者作为研究对象,宫颈慢性炎症105例,宫颈上皮内瘤变210例,宫颈癌74例。采用免疫组化方法检测宫颈组织中K-ras及C-myc蛋白的表达水平。结果宫颈癌C-myc、K-ras阳性表达率最高,其次为CINⅢ组,慢性炎症无阳性表达,组间比较差异有统计学意义(P<0.05)。低分化、有淋巴结转移、临床分期为Ⅲ~Ⅳ期的宫颈癌组织中C-myc、K-ras阳性率显著高于高分化、无淋巴结转移以及临床分期为Ⅰ~Ⅱ期的宫颈癌组织,差异有统计学意义(P<0.05)。相关性分析显示,C-myc蛋白与K-ras蛋白表达呈显著正相关的关系(r=0.683,P=0.000)。结论癌基因C-myc以及K-ras参与了宫颈癌的发生及发展过程,并且恶性程度越高,临床分期越晚,其阳性表达率越高。
Objective To explore the expressions and clinical significance of oncogenes K-ras and C-myc in cervical cancer. Meth- otis A total of 389 patients with abnormal TCT findings or abnormal HPV-DNA detection results were selected from the hospital from De- cember 2010 to June 2016 as study object, including 105 patients with chronic cervicitis, 210 patients with CIN, and 74 patients with cervi- cal cancer. The expression levels of K-ras and C-myc in cervical tissues were detected by immunohistochemical method. Results The pos- itive rates of C-myc and K-ras in cervical cancer group were the highest, followed by CIN Ⅲgroup, and there was no positive expression in chronic cervicitis group, which showed statistically significant differences （P〈0. 05 ） . The positive rates of K-ras and C-myc in low differ- entiated, lymph node metastasis, cervical cancer （stage Ⅲ-Ⅳ ） tissue were higher （P〈0. 05 ） . Correlation analysis showed that C-myc protein expression Was positively correlated with K-ras protein expression （ r = 0. 683, P = 0. 000 ） . Conclusion Oncogenes C-myc and K-ras are involved in the occurrence and development of cervical cancer, and the higher the degree of malignancy is, the later the clinical stage is, the higher the positive expression rate is.
出处
《中国妇幼保健》
CAS
2017年第9期2007-2010,共4页
Maternal and Child Health Care of China
基金
浙江省卫生和计划生育委员会科研项目(2015RCB032)
舟山市卫生局医药卫生科技计划项目(2013A11)
