摘要
目的观察不同浓度大黄对大鼠脊髓损伤(SCI)后血-脊髓屏障(BSCB)的影响,筛选大黄煎药灌服的最佳浓度,拟为临床治疗SCI提供一定的依据。方法成年健康SD大鼠125只,随机分为假手术组(Sham组)、模型组及大黄低、中、高剂量组。大黄各剂量组用大黄水溶液灌胃,其余两组用等量生理盐水。记录大鼠SCI后双后肢运动功能(BBB)评分、脊髓含水量以及荧光显微镜观察注入伊文思蓝(EB)后的BSCB情况。结果 (1)BBB评分:模型组和大黄低、中、高剂量组与Sham组比较差异均有统计学意义(P<0.05)。(2)脊髓含水量的测定:模型组和大黄低、中、高剂量组与Sham组比较差异均有统计学意义(P<0.05)。(3)荧光显微镜下观察到Sham组没有EB染出,模型组EB大量染出,以3 d时染出最多,大黄各剂量组EB染出较模型组有不同程度减少,以大黄中剂量组EB染出最少。结论中剂量组大黄有效地降低了大鼠SCI后BSCB的通透性,对其保护作用最为明显。
Objective To evaluate the effects of Rhubarb on blood spinal cord barrier in rats after spinal cord in- jury （SCI） , looking for a best concentration of Rhubarb decoction lavage and to provide a basis for clinical treatment of SCI. Methods A total of 125 adult healthy SD rats were randomly divided into sham operation group （Sham）, model group, and low, medium and high dose groups. Rhubarb solution was given in treatment groups, while normal saline as placebo was given in the other 2 groups. The neurological function of hind limbs was recorded using BBB score. The water content in spinal cord was observed. And using fluorescence microscope to observed the blood spinal cord barrier after in- jection of Evans blue （EB）. Results There were significant differences in BBB scores and water content in spinal cord between control group and model group, rhubarb low, medium and high dose groups （ P 〈 0. 05 ）. Fluorescence micro- scope showed that there was no EB stain in control group, while rich EB stain was revealed in model group. The EB stain in rhubarb groups showed reduction in different degreed compared with model group, especially in rhubarb medium dose group. Conclusion In the medium dose group, rhubarb can effectively reduce the permeability of the blood spinal cord barrier in rats after SCI.
出处
《广东医学》
CAS
北大核心
2017年第10期1481-1484,共4页
Guangdong Medical Journal
基金
国家自然科学基金面上项目(编号:81573997)
江苏省普通高校研究生科研创新计划项目(编号:KYLX16_1147)
徐州市2014年推动科技创新后补助备案项目(编号:KC14SH030)
关键词
脊髓损伤
大黄
血-脊髓屏障
spinal cord injury
rhubarb
decocting concentration
blood spinal cord barrier
