摘要
目的探讨miR-135b对子宫内膜癌中HOXA10基因表达的调控作用。方法 RT-PCR方法检测28例子宫内膜癌组织及相应癌旁组织中miR-135b及HOXA10的表达;用miR-135b模拟物及抑制物转染RL-952子宫内膜癌细胞株,RT-PCR检测转染效果并检测FOXO1 mRNA的变化,划痕实验检测转染后对细胞迁移的影响,流式细胞技术检测转染对细胞增殖的影响,Western blot检测HOXA10蛋白的变化。结果 miR-135b在子宫内膜癌组织中较对应的癌旁组织表达升高(P<0.05),HOXA10则降低(P<0.05),经miR-135b模拟物转染后,子宫内膜癌细胞中HOXA10 mRNA和蛋白的表达水平下降(P<0.05);经miR-135b抑制物转染后,子宫内膜癌细胞中HOXA10 mRNA和蛋白的表达水平升高(P<0.05);miR-135b能促进子宫内膜癌细胞的增殖(P<0.05)。结论 miR-135b在子宫内膜癌发生发展中有一定作用,子宫内膜中HOXA10的异常表达受miR-135b的调控。
Objective To explore the effects of miR - 135b on HOXA10 in endometrial cancer. Methods The expression of miR - 135b and HOXA10 in human endometrial cancer tissues and their para - carcinoma tissues was assessed by real - time PCR. miR - 135b mimics （ miR - 135b up - regulated expressions） and miR - 135b inhibitors （ miR - 135b down - regulated expression） were transfected into human endometrial cancer RL -952 cells by liposome method. The transfection efficiency was detected by RT - PCR. Wound healing assay was performed to detect the cell migration a- bility. Cell proliferation and cell cycle were analyzed 48h after transfection by flow cytometry. The expression of HOXA10 mRNA and protein were assessed by RT - PCR and Western blot. Results The expression of miR - 135b in human endometrial cancer tissues was significantly higher than their para - carcinoma tissues （ P 〈 0. 05 ）. Expression of HOXA10 in eudometrial carcinoma tissues was significantly lower than their para - carcinoma tissues （ P 〈 0. 05 ）. Compared with NC group, the mRNA and the protein expression of HOXA10 was significantly down- regulated in miR- 135b mimics group （ P 〈 0. 05 ）. However, in miR - 135b inhibitors group, the mRNA and the protein expression of HOXA10 was significant- ly up - regulated （ P 〈0. 05 ）. MiR - 135b can promote the proliferation of endometrial cancer cells （ P 〈 0. 05 ）. Conclusion MiR - 135b plays an important role in the occurrence and development of endometrial carcinoma by regulating HOXA10.
出处
《广东医学》
CAS
北大核心
2017年第9期1338-1342,共5页
Guangdong Medical Journal
基金
广东省科技计划项目(编号:2014A020212628)
