摘要
本文旨在探讨道地药材恒山黄芪的多糖成分对人宫颈癌细胞Si Ha生长和迁移能力的抑制效应。通过采用MTT法验证恒山黄芪多糖对Si Ha细胞生长活力的抑制效应,利用细胞黏附试验和铺展试验检测恒山黄芪多糖对Si Ha细胞的粘附能力和铺展能力的影响,并采用划痕试验检测恒山黄芪多糖对Si Ha细胞迁移运动能力的抑制效应。结果表明:恒山黄芪多糖具有抑制Si Ha细胞增殖的活性(IC50值为0.25 mg/m L),并且在低浓度(0.0625 mg/m L)时仍然能够有效抑制其生长;与对照细胞相比,恒山黄芪多糖处理的细胞在培养器皿底部的黏附能力和铺展能力显著下降(P<0.01),并且明显抑制了Si Ha细胞迁移运动能力(P<0.01)。因此,道地药材恒山黄芪的多糖成分具有抑制人宫颈癌细胞Si Ha的增殖、粘附、铺展以及迁移运动的活性。
To evaluate the effects of Hengshan Astragalus polysaccharides on human cervical cancer cell SiHa, MTT as- say was used to determine cell proliferation and viability of SiHa. The cell adhesive assay and cell spreading assay were used to detect the adhesive and spreading capabilities of SiHa, and the wound-healing assay was used to access the mi- gration capability of SiHa. The results indicated that Hengshan Astragalus polysaccharides showed significant activity of suppressing SiHa cell proliferation （ IC50 value was 0.25 mg/mL） even at a low concentration （0.0625 mg/mL）. Com- pared with the control group, the adhesive and spreading capability of SiHa cells treated with Hengshan Astragalus poly- saccharides were obviously reduced （ P 〈 0.01 ）, and the migration capability was also depressed remarkably （ P 〈 0.01 ）. Therefore, polysaccharides from the genuine medicinal material Hengshan Astragalus can inhibit the proliferation, adhesion, spreading and migration capability of human cervical cancer cell SiHa.
出处
《天然产物研究与开发》
CAS
CSCD
北大核心
2017年第4期653-657,共5页
Natural Product Research and Development
基金
山西省"服务产业创新学科群"培育项目
山西省高校创新学科群项目子项目(XKQZXM200601)
关键词
恒山黄芪
多糖
肿瘤细胞
粘附
铺展
迁移
Hengshan Astragalus
polysaccharides
cancer cells
cell adhesion
cell spreading
cell migration
