摘要
乳腺癌是一种严重威胁女性健康的恶性肿瘤,癌症干细胞假说的提出为乳腺癌的起因以及治疗提供了新的模型。对746个乳腺癌样本中的18409个基因和1035个miRNAs,通过生物信息学方法构建共表达网络,将其划分到不同的共表达模块中;利用胚胎干细胞和间充质干细胞的特性进一步筛选模块,得到两个大小分别为2019和859且与上述两类干细胞相关的基因集;最后通过构建这两个基因集的调控网络,筛选出两个胚胎干细胞的特异性关键基因TPX2和MCM10,以及间充质干细胞的特异基因COL5A2。这些基因可以作为癌症干细胞的候选特异性标志物,有望成为潜在的乳腺癌治疗标靶。
Breast cancer is a kind of malignant tumor which seriously threats the health of global female. However, the hypothesis of cancer stem cell （CSC） provides a new model for breast cancer causes and treatment. In the paper, coexpression network was constructed with the bioinformatics method for 18409 genes and 1oa5 miRNA in 746 breast cancer samples, and they were divided into different coexpression modules. The characteristics of embryonic stem cells and mesenchymal stem cells were utilized to further screen the modules, and two gene sets related to the above two types of stem cells （size. 2019 and 859） were gained respectively. Finally, regulatory network for the two gene sets were constructed to screen specific hub genes TPX2 and MCMIO of two embryonic stem ceils as well as specific gene COL5A2 of mesenchymal stem cells. These genes can be considered as candidate specific biomarkers of CSC and potential therapeutic targets in the treatment of breast cancer.
出处
《浙江理工大学学报（自然科学版）》
2017年第3期451-460,共10页
Journal of Zhejiang Sci-Tech University(Natural Sciences)
基金
国家自然科学基金项目(61170110
61272312)
浙江省自然科学基金项目(LY14F020049)
关键词
乳腺癌
胚胎干细胞
间充质干细胞
基因调控网络
关键基因
breast cancer
embryonic stem cells
mesenchymal stem cells
gene regulatory network
hub genes
