摘要
对发酵蔗渣产黄腐酸(FA)的高产菌株-枯草芽孢杆菌的培养基配方及培养条件进行优化研究.获得的优化培养基配方为:可溶性淀粉、蛋白胨、磷酸氢二钾和硫酸镁的添加量分别为12.50、12.50、0.25、0.50 g·L^(-1);优化的培养条件为:pH 7.0、温度37℃、装液量20 m L/250 m L、转速130 r·min^(-1)、接种量5%(体积分数).在该优化的培养基及培养条件下,枯草芽孢杆菌培养12 h后的细胞生物量达2.280×10~9cfu·m L^(-1),较优化前提高了77.9倍.采用优化前后的条件培养菌种进行发酵产黄腐酸实验,结果优化后的发酵产品中FA含量为26.36%(质量分数),细胞生物量为1.09×10~9cfu·g^(-1),比优化前分别提高了13.9%和2.3倍.
This study was performed to optimize the medium formula and culture conditions of Bacillus subtilis which produced fulvic acid (FA) from bagasse fermentation. The results showed that the opti-mal medium formula for Bacillus subtilis consisted of 12.50 g ? L 'soluble starch, 12.50 g·L^-1 pep-tone, 0.25g·L^-1 K2HPO 4 and 0.50g·L^-1 MgSO 4- And the optimal fermentation conditions were pH 7.0, 37℃ ,liquid volume of 20 mL/250 mL,rotation speed of 130 r·min^-1 ,and inoculation amout of 5%(volume fraction). Under these conditions after growing for 12 hours,the cell biomass of Bacillus subtilis reached 2.280x 109 cfu ? mL 1, which was increased by 77.9 times compared with those before the optimization. Applicated Bacillus subtilis which culture conditions were used before and after optimization to produce FA, the results showed that FA content in the fermentation product was 26.36%(mass fraction) and the cell biomass reached 1.09×10^9 cfu·g^-1,which were increased by 13.19% and 2.3 times compared with those before the optimization.
作者
谢航
邱宏端
林娟
赵莹
XIE Hang QIU Hongduan LIN Juan ZHAO Ying(College of Biological Science and Technology,Fuzhou University, Fuzhou,Fujian 350116,China)
出处
《福州大学学报(自然科学版)》
CAS
北大核心
2017年第3期438-445,共8页
Journal of Fuzhou University(Natural Science Edition)
基金
国家农业部2013年度农业公益性行业科研专项(201303094-05)
关键词
黄腐酸
枯草芽孢杆菌
培养条件优化
fulvic acid
Bacillus subtilis
culture conditions optimization