摘要
通过RT-PCR和RACE技术,从荔枝胚性愈伤组织中克隆得到了SUMO结合酶1基因的全长cDNA序列,命名为LcSCE1。LcSCE1 cDNA全长为840 bp,CDS全长483 bp,预测可编码160个氨基酸。生物信息学分析发现:LcSCE1为带负电的亲水不稳定蛋白,没有信号肽和跨膜结构域,进化上,LcSCE1与麻风树SCE1蛋白亲缘关系最近。利用Real-time PCR技术研究了LcSCE1在不同组织部位的表达情况,发现该基因在"元红"荔枝各组织器官中均有表达,在新叶与芽中表达量最高,膨大期果核中该基因的表达量也显著高于成熟果核(p<0.01)。此外,本研究还比较了该基因在醮核和正常核中LcSCE1的表达情况,发现它在醮核中的表达量显著高于正常核(p<0.01)。本研究结果表明LcSCE1可能在荔枝生长发育及醮核产生过程中发挥着重要作用。
In this study, a full-length eDNA sequence of SUMO-conjugating enzyme 1 gene （named as LcSCE1） was isolated from embryonic callus of Litchi chinensis cv. Yuanhong using RT-PCR and RACE methods. The complete eDNA sequence of LcSCE1 is 840 bp with a 483 bp long CDS, which is predicted to encode a protein of 160 amino acids. Bioinformatic analysis revealed that LcSCE1 was a stable and hydrophilic protein with no signal peptide and transmembrane domain. Phylogenetic tree analyses showed that LcSCE1 shared the highest similarity with Jatropha curcas SCE1. Quantitative real time PCR （qRT-PCR） results showed that expression of LcSCE1 can be found in all the tested tissues but its expression level differed a lot. The highest expression of LcSCE1 was found in the new leaves and buds, and the expression level of LcSCE1 in expanding seed was also significantly higher than that in normal mature seed （p 〈0.01）. Moreover, we investigated the expression levels of LcSCE1 in aborted-seeds and normal seeds, and very significant difference was found between the two kinds of seeds （p〈0.01）, Results generated in this study suggested that LcSCE1 might play important role in Litchi development as well as the emergence of aborted-seeds.
出处
《分子植物育种》
CAS
CSCD
北大核心
2017年第4期1249-1258,共10页
Molecular Plant Breeding
基金
福建省重大专项(2015NZ0002-1)
福建省科技重大专项(2013NZ0002)共同资助
