摘要
目的探讨野百合碱致肺动脉高压(PAH)大鼠IL-1β和IL-18表达的变化。方法将雄性大鼠随机分为对照组和实验组(n=10),建立野百合碱致PAH大鼠实验模型,应用B超、心肌细胞HE染色和TUNNEL染色检测;ELISA检测血清NF-κB、COX2、IL-6、IL-1β、TNF-α和NO浓度;免疫组化法检测肺组织IL-1β和IL-18表达;Western blot检测肺组织NLRP3蛋白表达。结果 PAH大鼠血清中NF-κB、COX2、IL-6、IL-1β、TNF-α和NO均显著增加(P<0.05);肺组织IL-1β和IL-18的表达明显增加(P<0.05),PAH大鼠肺组织IL-1β和IL-18阳性细胞百分比显著增加,IL-1β的增加更明显;NLRP3蛋白表达实验组比对照组有明显增加(P<0.05)。结论野百合碱致肺组织IL-1β和IL-18表达增加。
Objective Explore the change of IL-1β and IL-18 expression in pulmonary hypertension induced by monocrotaline.Methods Divide the mouses into two groups,control group and experimental group（n = 10）.Establish rats pulmonary hypertension model induced by monocrotaline.Detect the model by ultrasound,myocardial cells HE dyeing and tunnel test;ELISA was used to detect the serum biological markers NF-κB,COX2,IL-6,IL-1β,TNF-α and NO;Immunohistochemical was used to detect the expression level of IL-1β and IL-18 in the lung tissue;the protein change of NLRP3 in the lung tissue was detected by Western blot.Results Serum biological markers of NF-κB,COX2,IL-6,IL-1β,TNF-α and NO are significantly increased in PAH rats（P〈0.05）;The expression of IL-1β,IL-18 in the lung tissue increased obviously（P〈0.05）;The NLRP3 protein expression was significantly higher in experimental group.Conclusions Changes of NLRP3 effect increase expression of IL-1 β and IL-18 and which may play an important role in pulmonary hypertension induced by monocrotaline.
作者
张照奇
高戈
胡童谣
魏丹
袁衡
吴翔
郑涛涛
ZHANG Zhao-qi GAO Ge HU Tong-yao WEI Dan YUAN Heng WU Xiang ZHENG Tao-tao(Faculty of Laboratory Medicine, Xiangya Medical College, Central South University, Changsha 410013 Changsha Medical University, Changsha 410008 Faculty of Laboratory Medicine, Chinese Medicine Hospital of Hainan Province, Haikou 570100 Lixian Red Cross Hospital, Changde 415000, China)
出处
《基础医学与临床》
CSCD
2017年第5期687-690,共4页
Basic and Clinical Medicine
基金
湖南省自然科学基金(2015JJ4073)
中南大学硕士生自主探索创新项目(2016zzts501)
中南大学大学生创新创业项目(201510533375)
湖南省教育厅科学研究项目(15C0145)
海南省卫生厅普通科研课题(14a20026)
