摘要
目的分析低水平脂联素(APN)与胰岛素抵抗(IR)在心室重塑中的效应。方法将野生C57小鼠随机分为对照组(control组)和胰岛素抵抗组(IR组),每组n=8,脂联素基因敲除小鼠随机分为基因敲除组(APNKO组)和基因敲除+胰岛素抵抗组(APNKO+IR组),每组n=8,给予高脂饲料诱导产生IR。喂养12周后测定空腹血糖(FPG)、空腹胰岛素(FINS)、三酰甘油(TG)和总胆固醇(TC)等糖脂代谢指标,并计算各组胰岛素抵抗指数(HOMA-IR)、心脏质量指数(HWI)和左心室质量指数(LVWI)。取左心室心肌做Masson染色,计算心肌胶原容积分数(CVF)与血管周围胶原面积(PVCA)的大小,检测各组心肌纤维化的程度;用Western blot检测心肌基质金属蛋白酶-9(MMP-9)和APN表达量。结果低水平APN和IR均能增加心脏质量、左心室质量、HWI和LVWI(P<0.05)。低水平APN和IR对TG、TC、FPG、FINS及心室重塑相关指标有交互效应(P<0.05),且均为正效应,对心肌APN也有交互效应(P<0.05),但为负效应。结论低水平APN和IR在小鼠心室重塑过程中起协同促进作用。
Objective The study the effect on cardiac remodeling induced by low level adiponectin（APN） and insulin resistance（IR）.Methods 16 wild-type C57 mice were randomly divided into control and IR groups.16 APN gene knockout mice were randomly divided into APNKO and APNKO ＋ IR groups.The IR and APNKO ＋ IR groups were a high-fat feed to induce insulin resistance.After 12 weeks,cholesterol（TC）,triglycerides（TG）,fasting plasma glucose（FPG）,fasting insulin（FINS） were measured.HOMA-IR,HWI and LVWI were calculated.The left ventricular myocardial were examined by microscopy to observe the degree of myocardial fibrosis,and the protein expression of matrix metalloproteinase-9（MMP-9） and APN in myocardial were detected with western blot.Results Low level APN and IR increased heart weight,left ventricular weight,HWI,LVWI（P〈0.05）.There were interaction between IR and low level APN on the TC,TG,FPG,FINS,and the index related myocardial fibrosis（P〈0.05）,and they were positive effect.There was also interaction effect between IR and low level APN on myocardial APN expression,but it was the negative effects（P〈0.05）.Conclusions Both low level adiponectin and insulin resistance could lead to cardiac remodeling synergistically.
作者
徐璐
刘雅娟
张青
张娜
任永康
杨锐英
XU Lu LIU Ya-juan ZHANG Qing ZHANG Na REN Yong-kang YANG Rui-ying(Dept. of Cardiovascular Medicine, Ningxia Medical University, Yinchuan 750000 Heart Centre, General Hospital of Ningxia Medical University, Yinchuan 750000, China)
出处
《基础医学与临床》
CSCD
2017年第5期625-629,共5页
Basic and Clinical Medicine
基金
国家自然科学基金(81360026)
关键词
胰岛素抵抗
脂联素
心室重塑
insulin resistance
adiponectin
cardiac remodeling
