摘要
目的通过向10d龄乳鼠缺血缺氧模型注入USPIO标记人脐带间充质干细胞(MSCs),利用3.0TMR监测MSCs进入鼠体内后的生存、转化、迁移,探索MSCs移植活体示踪技术;观察MRI图像变化及神经学评分,初步探讨MSCs对乳鼠缺氧缺血性脑病(HIE)的治疗效果,并分析其治疗机制。方法培养MSCs,以多聚赖氨酸(PLL)为转染剂,用USPIO对MSCs-PLL进行磁性标记;将磁标记的MSCs通过鼠尾静脉移植入HIE乳鼠体内,分别于移植前、移植后1d,10d行MRI观察及神经学评分(NSS评分)。结果 1USPIO-PLL可安全有效标记MSCs,标记率大于96%,细胞活性无影响;2移植后1d MRI可监测到MSCs到达病变区,10d时到达病变区MSCs增多,MRI信号降低(60.66±15.28)%(P<0.05);建模成功后,MSCs组移植后1d、移植后10d脑梗死体积相对体积组间差异有统计学意义(P<0.05);NS组(生理盐水对照组)差异无统计学意义(P>0.05);MSCs组与NS组移植10d脑梗死体积相对体积的变化值比值差异有统计学意义(P<0.05)3MSCs移植组、NS组移植10d后及移植前神经病学评分分别为(3.2±2.1),(8.0±1.9),(8.1±1.8)分,组间差异有统计学意义(P<0.05)。结论 MRI可以有效进行干细胞移植后的活体示踪;干细胞对缺血缺氧梗死区有定向趋化作用,可以促进受损神经恢复而起到治疗作用;为新生儿HIE临床治疗提供了有力的实验依据。
Objective To investigate the survival ,transformation and migration of MSCs in vivo by injecting USPIO-labeled umbilical cord mesenehymal stem cells （MSCs） into the rat model of hypoxia and hypoxia for 10 days, and using 3.0 TMR to observe MSCs transplantation. To observe the MRI image changes and neurological score,preliminary study of MSCs on neonatal HIE treatment, analysis of treatment mechanism. Methods MSCs-PLL were labeled with USPIO; tbe magnetic labeled MSCs were transplanted into the HIE milk through the tail vein of the mice. The MSCs-PLL was labeled with poly-L-lysine（PLL） as the transfection reagent. MRI and neurological score （NSS） were performed before transplantation and 1 d and 10d after transplantation. Results （1） USPIO-PLL could safely and effectively label umbilical cord mesenchymal stem cells with a labeling rate of more than 96% and no effect on cell viability;（2）One day after transplantation, MRI could detect mesenchymal stem cells reaching the lesion area（60.66 ± 15.28 ）% ,P 〈 0.05 ;After successful modeling, MSCs group 1d after transplantation,10d after transplantation, the relative volume of cerebral infarction volume difference between groups were statistically significant（ P 〈 0.05）. There was no significant difference in the relative volume of cerebral infarction volume between the MSCs group and the NS group （ P 〈 0.05 ）. （3）The neurological scores of MSCs transplantation group and NS group were 3.2± 2.1 , 8.0 ± 1.9 and 8.1±1.8 respectively after transplantation for 10 days ,and there was significant difference between the two groups （P 〈 0.05）. Conclusion MRI can effectively trace the stem cells after transplantation;the stem ceils have a directional chemotaxis effect on ischemic infarct area, which can promote the recovery of injured nerves and play a therapeutic role;It provides a powerful experimental basis for the clinical treatment of neonatal HIE.
出处
《潍坊医学院学报》
2017年第2期135-138,共4页
Acta Academiae Medicinae Weifang
基金
潍坊市卫计委资助课题(课题编号:2013-012)
关键词
间充质干细胞
缺氧缺血脑病
USPIO
Mesenchymal stem cells （ MSCs ）
Hypoxic-ischemic eneephalopathy （ HIE ）
USPIO
