摘要
RNA干扰(RNA interference,RNAi)技术具有专一性强、抑制效率高、可大规模操作、重复性好及在植物中可以孟德尔方式遗传等优点,被广泛应用于基因功能验证、代谢调控等方面。在植物代谢工程中,可通过RNAi方法抑制分支途径产物合成及改变代谢流的分配,调控目标物质的合成。对RNAi技术发生机制、高效RNAi载体构建方法及其在代谢调控中的应用进展进行系统概述,以期为利用RNAi技术进行代谢工程调控研究奠定理论基础和提供技术参考。
RNA interference （RNAi） technology has strong specificity, high inhibitory efficiency, large-scale operation application, and good repeatability, and it can perform Mendelian mode of inheritance in plants, which is widely used in the validation of gene function and metabolic regulation. In plant metabolic engineering, the RNAi method can inhibit the synthesis of product, change the distribution of metabolic flux, and regulate the synthesis of the target product. This review summarized the current progress and study strategy of RNAi in plant metabolic regulation. RNAi mechanism and characteristics, optimal strategy of vector structure, efficient RNAi vector construction method, and application progress in the regulation of metabolism were reviewed, in order to lay the theoretical foundation and technical reference of RNAi technology of metabolic engineering control.
出处
《中草药》
CAS
CSCD
北大核心
2017年第7期1458-1465,共8页
Chinese Traditional and Herbal Drugs
基金
东北林业大学本科创新课题(201510225155)
国家自然科学基金资助项目(31570589)
中央高校基本科研业务费资助(257-2015DY01)
关键词
RNAI
代谢调控
载体构建
次生代谢
植物代谢工程
RNAi
metabolic regulation
vector construction
secondary metabolism
plant metabolic engineering
