摘要
目的建立一种适合于工业化生产的甘草总黄酮提取和纯化方法。方法以甘草总黄酮的提取率为指标,采用正交试验确定氨水提取的最佳条件;并以甘草总黄酮的转移率和除杂率为指标,采用正交试验优化最佳超滤工艺参数。结果最佳提取条件:0.75%氨水24倍,提取3次,每次60 min,总黄酮平均提取率达83.5%;超滤结果表明:选用10 nm的无机陶瓷膜,在操作压力0.12 MPa和料液温度25℃条件下甘草总黄酮平均转移率和平均除杂率分别达到95.6%和23.3%。结论该联用技术简便可行,生产成本低,适合工业化生产。
OBJECTIVE To establish a suitable method for industrialized production of total flavonoids extraction and purification from Glycyrrhizae Radix et Rhizoma. METHODS The orthogonal test was used to determine the best extraction conditions within the extraction rate of total flavonoids as the index; and orthogonal experiment was used to optimize the parameters of ultrafiltration process taking the transfer rate of total flavonoids and the ratio of removal of impurity as indexes. RESULTS The best extraction conditions were as follows： 24 times of 0.75% ammonia, extract 3 times, 60 rain for each time, the average extraction rate of total flavonoids was 83.5%. Ultrafiltration results showed that： 10 nm of inorganic ceramic membrane, in the operating pressure of 0.12 MPa and liquid temperature of 25 ℃, the average transfer rate and average removal rate of total flavonoids were 95.6% and 23.3%. CONCLUSION The combined technique is simple and feasible, and the production cost is low, the method is suitable for industrial application.
出处
《中国现代应用药学》
CAS
CSCD
2017年第4期492-495,共4页
Chinese Journal of Modern Applied Pharmacy
基金
国家自然科学基金项目(81060345
81460608)
甘肃省基础研究创新群体项目(1506RJIA034)
关键词
甘草
总黄酮
提取
超滤
转移率
除杂率
Glycyrrhizae Radix et Rhizoma
total flavonoids
extraction
ultrafiltration
transfer rate
removal rate
