摘要
采用乌氏黏度计测定不同pH条件下牛血清白蛋白(BSA)的黏度,运用Wolf方程描述BSA的黏度变化行为.详细讨论了不同pH条件下BSA的特性黏数(intrinsic viscosity,[η])与其构象的关系,并分析了BSA比特性黏数(specific intrinsic viscosity,{η})的组成变化.研究结果表明,采用这种新型的黏度法研究球状蛋白质的构象或溶液性质是科学、可行的.
The viscosities of bovine serum albuminin （BSA） in different pH buffers were measured using Ubbelohde capillary vis-cometer,and the data were evaluated by Wolf equation to describe the composition dependences of BSA. The intrinsic viscosities（[η]） of BSA under different pH were correlated with its conformation,and the composition dependences of specific intrinsic viscosi-ties （[ η] ） of BSA were carefully analyzed.Our results indicate that the new viscometric method can be applied in the study of conformarion and solution properties of spherical protein.
出处
《厦门大学学报（自然科学版）》
CAS
CSCD
北大核心
2017年第3期456-460,共5页
Journal of Xiamen University：Natural Science
基金
国家自然科学基金(51273166)
关键词
黏度法
特性黏数
牛血清白蛋白
构象
viscometric method
intrinsic viscosity
bovine serum albumin（BSA）
conformation
