摘要
目的评价分子线性探针杂交方法(LPA)在快速检测耐多药结核病人耐药性的应用价值。方法利用分子线性探针杂交方法检测415例结核分枝杆菌临床分离株,同时使用常规药敏方法对样本进行抗结核药物敏感性测试。结果线性探针杂交法检测415例结核分枝杆菌临床分离株RFP和INH耐药性的灵敏度、特异性分别为91.43%、94.20%和75.68%、95.89%,两种检测方法的kappa值分别为0.830、0.731;线性探针杂交法检出MDR的敏感度和特异度分别为75.44%、95.25%,两种检测方法的kappa值为0.692。线性探针杂交法检测的耐药株中RFP常见的突变型主要分布在WT7、WT8和MUT3(S531L)。结论线性探针杂交法在结核病耐药分析中能够快速、准确的鉴定出RFP和INH的耐药性以及常见耐药基因型,能够大幅缩短药敏试验时间,便于及时用药治疗,对控制耐药结核病的传播有重要意义。
Objective To evaluate the application of molecular linear probe hybridization method （LPA） in rapid detection of multi - drug resistant tuberculosis. Methods It used LPA to detect 415 cases of mycobacterium tuberculosis clinical isolates. At the same time, it also used conventional DST method to detect the samples. Results The sensitivity and specificity of RFP and INH clinical isolates was 91. 43% and 94. 2%, and 75. 68% and 95. 89% respectively by LPA method, and the value of kappa was 0. 830 and 0. 731 for the two methods. The sensi- tivity and specificity of MDR by PLA method was 75. 44% and 95. 25% respectively, and the kappa value was O. 692. The mutant resistant strains of RFP mainly distributed in WT7, WT8 and MUT3 （$531L）. Conclusion LPA method can detect RFP and INH drug resistance and other common resistant genes rapidly and accurately, which means it can sharply reduce the susceptibillty test time for timely clinical drug treatment.
作者
包训迪
王超
阚晓红
王庆
BAO Xun-di WANG Chao KAN Xiao-hong WANG Qing(Department of Labrtory, Anhui Chest Hospital, Hefei, Anhui 230022, Chin)
出处
《临床肺科杂志》
2017年第6期976-979,共4页
Journal of Clinical Pulmonary Medicine
基金
安徽省卫计委课题(No 15tb011)
安徽省胸科医院课题(No xk2015010)
