摘要
背景:外科手术是治疗脊柱结核的重要手段。但考虑脊柱位置的特殊性,承受机体应力及需拆除内固定所致二次损伤是外科治疗的局限所在。组织工程为各种疾病的治疗带来了新的思路和希望。理想生物材料代替传统内固定材料,具有明显无毒害及明显免疫排斥反应。目的:探究海藻酸盐结合骨髓间充质干细胞修复脊柱结核的力学性能。方法:将40只新西兰兔等分为对照组、脊柱结核组、海藻酸盐组与钛合金组。对照组不予以任何处理,其他组于L_5近椎间盘处钻孔填充明胶海绵,注射结核菌混悬液0.1 mL(菌量5 g/L),建立脊柱结核模型。2个月后,后2组模拟L_(4-5)脊柱结核切除,植入复合骨髓间充质干细胞的藻酸盐凝胶材料和钛合金内固定材料。结果与结论:钛合金组1只新西兰兔出现局部感染、红肿;海藻酸盐组未出现局部感染、红肿等不良反应。海藻酸盐组及钛合金组前屈、后伸及侧弯距离及最大轴向拔出力均优于脊柱结核组,均接近于对照组。结果说明复合骨髓间充质干细胞的藻酸盐凝胶材料植入后与机体生物相容性较好,且复合骨髓间充质干细胞的藻酸盐凝胶材料植入后脊柱稳定性与钛合金接近,具有良好的脊柱稳定性。
BACKGROUND： Although considerable progress has been made in spinal tuberculosis surgery,considering the particularity of the spine position, the surgical treatment is limited by the body stress and secondary injury due to removal of the internal fixation. Tissue engineering provides a new insight into the treatment of various diseases.Ideal biomaterials instead of traditional internal fixation materials are characterized by non-toxic and non-immune rejections.OBJECTIVE： To explore the biocompatibility and mechanical properties of alginate combined with bone marrow mesenchymal stem cells for repair of spinal tuberculosis.METHODS： Forty New Zealand rabbits were divided into four groups, 10 rats in each group： control group, spinal tuberculosis group, alginate group and titanium alloy group. The control group received no treatment, and in the other groups, boreholes were drilled in the fifth lumbar intervertebral disc and filled with gelatin sponge, and Mycobacterium tuberculosis suspension 0.1 mL （the amount of bacteria, 5 g/L）was then injected to make spinal tuberculosis models.Two months after modeling, simulated L4-5 spinal tuberculosis surgery was performed in the latter two groups, and alginate combined with bone marrow mesenchymal stem cells and titanium alloy internal fixation material were implanted,respectively.RESULTS AND CONCLUSION： One rabbit in the titanium alloy group had local infection and swelling. No rabbit in the alginate group had adverse reactions, such as swelling and local infection. Anteflexion, rear protraction, lateral bending distance and maximum axial pullout force in the alginate group and titanium alloy group were superior to those in the spinal tuberculosis group （P 〈 0.05）, which were close to normal levels （P 〉 0.05）. The above results showed that the alginate gel combined bone marrow mesenchymal stem cells has good biocompatibility with the body after implantation,and the post-implantation spinal stability is good and close to that after implantation of th
出处
《中国组织工程研究》
CAS
北大核心
2017年第10期1565-1570,共6页
Chinese Journal of Tissue Engineering Research
基金
泸州市科技计划项目(2012-S-40(1/5))
四川省卫生厅科研课题(120389)~~
