摘要
目的探讨红曲素(monascin)在脑出血后脑保护作用的机制及最佳剂量。方法 2016年3月—2016年10月于山西医科大学神经电生理实验室选取雄性SD大鼠120只,随机分为假手术组、模型组、低剂量红曲素组、中剂量红曲素组、高剂量红曲素组各24只。采用Ⅳ型胶原酶定位注射制备大鼠脑出血模型,于术后1、3、7、14 d行Garcia神经功能缺损评分,行脑水含量测定、铁染色及PPAR-γ、Nrf2、SRA测定。结果与假手术组相比,模型组、低剂量组、中剂量组及高剂量组神经功能缺损评分在1、3、7、14 d显著降低(F=181.705、494.229、226.576、24.726,P<0.01),且在1、3 d评分最低,脑水含量在1、3 d显著增高(F=21.781、70.036,P<0.05),铁沉积颗粒量在3、7、14 d显著增多(F=21.426、118.122、107.773,P<0.05),且于7~14 d达高峰,PPAR-γ免疫阳性细胞数于1、3、7 d显著增多(F=3.394、28.622、14.12,P<0.05),在3~7 d达高峰,Nrf2免疫阳性细胞数于1、3、7、14 d增多(F=33.076、23.17、47.779、12.182,P<0.05),SRA免疫阳性细胞数于1、3、7、14 d显著增多(F=22.283、511.092、51.811、31.060,P<0.05)。与模型组相比,高剂量组Garcia评分在3~7 d时升高(P<0.05),脑含水量及铁染色颗粒量降低(P<0.05),高剂量组PPAR-γ、Nff2及SPA免疫阳性细胞数于1、3及7 d明显升高(P<0.05),3~7 d达高峰,14 d降至模型组水平。结论高剂量红曲素可增加脑出血后PPAR-γ、Nrf2及SPA阳性细胞数,减轻脑出血后脑水肿及脑铁含量,改善脑出血后神经功能缺损。
Objective To investigate the protective effect of monascin on cerebral hemorrhage and its optimal dose.Methods From March 2016 to October 2016 at the Shanxi Medical University neural electrophysiology laboratory selected120 male SD rats were randomly divided into sham operation group,model group,low dose monascin group,middle dose monascin group and high dose monascin group with 24 rats in each group.The rat model of cerebral hemorrhage was established by injection of Gollagenase type Ⅳ.The neurological deficit scores of Garcia were measured at 1,3,7 and 14 d after operation.Results Compared with the sham group,the scores of neurological deficits in model group,low dose group,middle dose group and high dose group were significantly decreased at 1,3,7 and 14 days（F = 181.705,F =494.229,F =226.576,F =24.726,P 〈0.01）.The brain water content was significantly higher at 1 and3 d,respectively（F =21.781,70.036,P 〈 0.05）.The amount of iron deposition was significantly increased at 3,7 and 14 days（F = 21.426,118.122,107.773,P 〈0.05）,and reached the peak at 7-14 days,and the number of PPAR-γ immunoreactive cells increased significandy at 1,3 and 7 days（F =3.394,F =28.622,F =14.12,P 〈0.05）（F =33.076,F =23.17,F =47.779,F =12.182,P 〈 0.05）.The number of SRA immunoreactive cells was significandy higher than that of the control group（F =22.283,F =511.092,F =51.811,F =31.060,P 〈0.05）.Compared wiuS the model group,the Garcia score of the high dose group increased（P 〈0.05）,the brain water content and the amount of iron stained particles decreased（P 〈0.05）,and the high dose group PPAR-γ,Nrf2 and SRA The number of positive cells was significantly increased at 1,3 and 7 days（P 〈 0.05）,reached the peak at 3-7 days and decreased to the level of model group at 14 days.Conclusion High dose of monascus can increase the number of PPAR-γ,Nrf2 and SRA positive cells after cerebral hemorrhage,reduce brain edema and brain iron content after cerebral hemorrhage,and improve neurological
作者
伊晋莹
王改青
王娟
段淑娜
李曈
董梁
YI Jinying WANG Gaiqing WANG Juan DUAN Shuna LI Tong DONG Liang(Graduate School, Shanxi Medical University, Taiyuan 030000, Chin)
出处
《疑难病杂志》
CAS
2017年第5期500-504,I0002,共6页
Chinese Journal of Difficult and Complicated Cases
基金
山西省基础研究计划项目(2014011041-5)
2014年山西省卫生计生委科研项目(201301010)
关键词
脑出血
红曲素
脑水肿
神经功能缺损
大鼠
Intracerebral hemorrhage
Monascin
Brain edema
Neurological deficit
Rats
