摘要
目的 研究电磁噪声对 5 0Hz工频磁场诱导细胞内应激活化蛋白激酶 (stress activatedproteinkinase ,SAPK)磷酸化的影响 ,探索电磁噪声对极低频磁场生物效应可能存在的干预作用。方法 中国仓鼠肺成纤维细胞 (CHL)分别经 0 .4mT工频磁场、等强度的电磁噪声、工频磁场与噪声的复合磁场辐照及假辐照处理 3min和 15min后 ,裂解细胞 ,提取细胞蛋白质 ,以Western印迹技术测定并比较细胞内SAPK磷酸化 (活化 )的变化。结果 0 .4mT的工频磁场可诱导细胞内SAPK的磷酸化。经 3min及 15min辐照后 ,磷酸化SAPK含量分别增至 4 9.3%及 5 7.0 % ,与假辐照组比较 ,差异有显著性 (P <0 .0 5 ) ,而相同强度的电磁噪声不能增强SAPK的磷酸化 ,磷酸化SAPK分别为 37.7%及31.8% ,与假辐照组比较 ,差异无显著性 (P >0 .0 5 )。复合磁场辐照 3min后 ,可明显抑制工频磁场对SAPK磷酸化的增强作用 ,磷酸化SAPK含量下降至 2 4 .4 % ,与辐照组相比 ,差异有显著性 (P <0 .0 5 ) ;辐照 15min后也可降低工频磁场对SAPK磷酸化的诱导作用 ,磷酸化SAPK含量下降至 39.0 % ,与工频磁场组和假辐照组相比 ,差异均无显著性 (P >0 .0 5 )。结论 一定强度的电磁噪声可以抑制工频磁场诱导SAPK磷酸化 。
Objective To investigate the possible interference effect of electromagnetic noise exposure on phosphorylation of stress activated protein kinase(SAPK) induced by 50 Hz magnetic field(MF). Methods Chinese hamster lung(CHL) cells were exposed to sham exposure(C),0.4 mT 50 Hz sinusoidal MF,0.4 mT electromagnetic noise and the combined noise MF with 50 Hz MF for 3 min and 15 min respectively.After exposure,the cells were lysed,and the proteins were extracted.The SAPK and phosphorylated SAPK(activated form of SAPK) were measured indirectly by Western blot with corresponding antibodies.The percentage of phosphorylated SAPK was calculated and analyzed. Results Exposure of cells to 50 Hz MF for 3 min and 15 min enhanced the SAPK phosphorylation.The percentage of phosphorylated SAPK were 49.3% and 57.0% respectively,and were significantly different from those of control( P <0.05, n =4).However,single noise MF exposure with the same intensity did not enhance the SAPK phosphorylation,the percentage of phosphorylated SAPK were 37.7% and 31.8%( P >0.05).When cells were exposed to the combined noise MF with 50 Hz MF for 3 min,the SAPK phosphorylation was significantly inhibited(24.4%,P<0.05);for 15 min,the SAPK phosphorylation was also decreased (39.0%), but there was no significant difference from control and 50 Hz MF exposure(P>0.05). Conclusion Noise MF with certain intensity could inhibit the biological effect induced by 50 Hz MF.
出处
《中华劳动卫生职业病杂志》
CAS
CSCD
北大核心
2002年第4期246-248,共3页
Chinese Journal of Industrial Hygiene and Occupational Diseases
基金
国家自然科学基金资助项目 (5 0 13 70 3 0
3 0 10 0 0 3 6)
关键词
磷酸化
工频磁场
电磁噪声
应激活化蛋白激酶
Power frequency magnetic fields
Electromagnetic noise
Stress activated protein kinase(SAPK)
