摘要
为研究促肾上腺皮质激素释放激素结合蛋白(corticotropin-releasing hormone binding protein,CRHBP)作为番鸭异常行为研究候选基因的可能性。实验以半番鸭为实验材料,设计9对引物对CRHBP基因内含子和外显子序列进行扩增,其PCR产物经测序后,利用生物信息学软件分析SNPs位点突变前后CRHBP基因及其蛋白结构的变化。结果表明,在扩增的CRHBP基因中筛选出12个SNPs:Exon3-G^(5805)A、Exon3-C^(5906)T、Exon7-A^(10747)G、Intron3-A^(5984)G、Intron3-T^(6019)G、Intron3-C^(6021)T、Intron3-G^(6035)A、Intron3-G^(6036)A、Intron7-C^(10506)T、Intron7-A^(10613)G、Intron8-G^(12097)A和Intron8-G^(12118)A,其中Exon3-G^(5805)A和Exon7-A^(10747)G为同义突变;Exon3-C^(5906)T是错义突变,导致编码的缬氨酸(Val)突变为异亮氨酸(Ile);Intron3-A^(5984)G、Intron3-T^(6019)G、Intron3-C^(6021)T、Intron3-G^(6035)A、Intron3-G^(6036)A、Intron7-C^(10506)T、Intron7-A^(10613)G、Intron8-G^(12097)A和Intron8-G^(12118)A处于内含子区域,不参与氨基酸编码。
To study the possibility of corticotropin-releasing hormone binding protein （CRHBP） as a candidate gene for Cairina moschata abnormal behavior study, the research took Cairina moschata as experimental material and designed 9 pairs of primers to amplify the intron and exon sequence of CRHBP gene. After the sequencing of PCR product, bioinformatics software was used to analyze the changes of CRHBP gene and its protein structure before and after SNPs mutation. The results showed that 12 SNPs were screened out from the amplified CRHBP gene： Exon3-G^5805A、Exon3-C^5906T、Exon7-A^10747G、Intron3-A^5984G、Intron3-T^6019G、Intron3-C^6021T、Intron3-G^6035A、Intron3-G^6036A、Intro n7-C^10506T、Intron7-A^10613G、Intron8-G^12097A和Intron8-G^12118A,其中Exon3-G^5805A和Exon7-A^10747G were synonymous mutations; Exon3-C59~6T was missense mutation and it resulted in the mutation of the encoded valine （Val） into isoleucine （Ile）; Intron3-A5984G、Intron3-T^6019G、Intron3-C^6021T、Intron3-G^6035A、Intron3-G^6036A、Intron7-C^10506T、Intron7-A^10613G、Intron8-G^12097A, and Intron8-G^12118A were in the introns area, so they did not participate in encoding amino acids.
出处
《基因组学与应用生物学》
CAS
CSCD
北大核心
2017年第4期1492-1497,共6页
Genomics and Applied Biology
基金
国家自然科学基金(31360566)
贵州省教育厅产学研基地项目(黔教合KY字2013-120号)
贵州省科技厅农业重大专项[黔科合重大专项字(2012)6004号]基金共同资助
