摘要
黄花蒿(Artemisia annua L.)是菊科(Asteraceae)蒿属(Artemisia)一年生草本植物。WD40转录因子基因与植物叶片表面腺毛发育、次生代谢相关。本研究利用RACE技术从黄花蒿中克隆出Aa WD40基因(GenBank登录号:KY411922),并对其进行了生物信息学分析。AaW D40基因的cDNA全长1 498 bp,其中5'末端非翻译区为209 bp,3'末端非翻译区为183 bp。开放阅读框(ORF)的长度为1 106 bp,编码368个氨基酸残基,DNAMAN分析预测其等电点为4.64,分子量为41.29 kD。对AaW D40氨基酸序列进行同源性分析,发现其与多种植物具有较高的同源性。生物信息学分析发现AaW D40氨基酸序列含有4个典型的WD40重复区域,符合WD40基因的特征,所以命名为AaW D40。利用Swiss-Model对AaW D40蛋白进行三级结构分析,AaW D40蛋白空间结构错综复杂,与多种植物WD40空间结构域非常相似。系统发育树分析AaW D40与其他WD40均源于同一祖先,并且AaW D40与DpWDR、GhTTG4的亲缘关系最为接近。荧光实时定量PCR分析显示,AaW D40在根、茎、叶中都可以检测到表达,其中根的表达量最高,其次为叶,再次为茎。本研究为进一步研究AaW D40与黄花蒿次生代谢的关系提供了理论基础。
Artemisia annua is an annual herb plant that belongs to Asteraceae, Artemisia. Transcription factor WD40 is involved in the development of glandular hairs on the leaf surface and secondary metabolism. This study cloned A αWD40 gene （GenBank Accession No. KY411922） from A rtemisia annua by RACE technology and ana- lyzed its bioinformatics. The full length of cDNA inAαwD40 was 1 498 bp, of which there were 209 bp in 5＇ untr anslated region and 183 bp in 3＇ untransled region. In addition, the length of open reading frame （ORF） was 1 106 bp, encoding 368 amino acid residues with the isoelectric point of 4.64 and molecular weight about 41.29 kD through the analysis and prediction of DNAMAN. The results of homology analysis of amino acid sequence in AaWD40 showed that it had high homology with many other plants. Bioinformatics analysis suggested that amino acid sequence of AaWD40 contained 4 typical WD40-repeat motifs, which conformed to the characteristics of the WD40 gene, so it was named A aWD40. In tertiary structure analysis of AaWD40 by Swiss-Model software showed that the spatial structure of AaWD40 protein was complex, which was quite similar with the WD40 space structure domain of other plants. Phylogenetic tree analysis revealed that AaWD40 and other WD40s originated from the same, and AaWD40 had the closest relationship with DpWDR and GhTTG4. Real-time PCR analysis told that the expression ofA alVD40 could be detected in roots, stems and leaves, with the highest expression in roots, followed by leaves and stems in turn. This stusy provided theoretical basis for the further study of the relationship between A αWD40 and the secondary metabolism ofA. annua.
出处
《基因组学与应用生物学》
CAS
CSCD
北大核心
2017年第3期1001-1008,共8页
Genomics and Applied Biology
基金
江苏省普通高校研究生科研创新计划项目(CXLX13-841)
苏州大学东吴学者项目(14317363)
国家自然科学基金项目(81273487)共同资助
关键词
黄花蒿
AαWD40
生物信息学分析
组织表达分布
A rternisia armua, AαWD40, Bioinformatic analysis, Distribution of tissue expression
