摘要
以红树莓品种海尔特兹和秋福的单芽茎段外植体为试验材料,研究了树莓组培快繁各阶段的最佳培养基及出瓶移栽基质。结果表明,初代培养基为MS+6-BA 0.1 mg/L时两个红树莓品种的诱导腋芽萌发效果最好,MS+6-BA 0.5 mg/L+NAA 0.1 mg/L为最佳继代增殖培养基。海尔特兹的最佳生根培养基为1/2MS+IBA 0.1 mg/L+NAA 0.05 mg/L,秋福的最佳生根培养基为1/2MS+IBA 0.2 mg/L+NAA 0.1mg/L。最佳出瓶移栽基质为蛭石、河沙、育苗基质、园土按体积比0.15∶0.15∶0.2∶0.5配制。
For the purpose of rapid propagation of 2 cultivars of red raspberry Heritage and Autumn Bliss, media of each propagating stage in vitro and transplanting in soil are optimized and compared. Single-bud-stem cuttings of the two cultivars are used as initial explants in this research. The result shows that the highest initial sprouting rates are achieved with the both varieties on a MS medium supplemented with 6-BA at 0.1 mg/L; for shoot multiplication, a medium composition as MS＋6-BA 0.5 mg/L＋ NAA 0.1 mg/L is the optimal. The best rooting medium for Heritage is 1/2MS＋IBA 0.1 mg/L＋NAA 0.05 mg/L, while the highest rooting ratio for Autumn Bliss was observed on a medium 1/2MS＋IBA 0.2 mg/L＋NAA 0.1 mg/L. Shoots developed good roots invitro are then transplanted into soil and a mixture of 15% vermiculite ＋15% sand＋20% nursery matrix＋50% garden soil is the best transplanting matrix.
作者
夏瑞英
岳雷
陆璐
柳永霞
王赟
成艳玲
余倩倩
杨伟
XIA Ruiying YUE Lei LU Lu LIU Yongxia WANG yun CHENG Yanling YU Qianqian YANG Wei(Jiuquan Jinshuoyuan Modem Agricultural Development Limited by Share Ltd., Gansu Jiuquan 735000, China Institute of New Rural Development Research, Northwest Normal University, Lanzhou Gansu 730070, China Characteristics of Plant Effective Components of Product Engineering Technology Research Center, Lanzhou Gansu Lanzhou 730070, China College of Horticulture, South China Agricultural University, Guangzhou Guangdong 510642, China)
出处
《甘肃农业科技》
2017年第4期16-19,共4页
Gansu Agricultural Science and Technology
关键词
红树莓
海尔特兹
秋福
组培快繁
Red raspberry
Heritage
Autumn Bliss
Tissue culture and rapid propagation
