摘要
应用介质阻挡放电(Dielectric barrier discharge,DBD)等离子体处理有毒有害蓝藻,并对蓝藻细胞损伤过程及藻毒素降解效率进行研究。以三维荧光光谱(Three dimensional excitation emission matrix fluorescence spectroscopy,EEM)技术为手段,通过区域荧光体积积分(Fluorescence regional integration,FRI)的方法,考察了介质阻挡放电等离子体损伤铜绿微囊藻(Microcystic aeruginosa)过程中藻细胞荧光类内含物释放降解的规律;此外,还利用高效液相色谱(HPLC)技术,检测了灭藻过程中微囊藻毒素(Microcystin-LR,MC-LR)含量的动态变化过程。结果表明,随着放电等离子体处理时间的延长,藻细胞内含物先释放到细胞外后,然后逐渐被降解;分析了蓝藻毒素MC-LR的变化过程与降解效率,证明它最终可以被等离子体完全氧化降解和去除。研究工作不仅显示了采用低温等离子体可以有效灭藻和降解藻毒素,同时也展示了三维荧光光谱作为一种快速实时的观察分析手段,可以有效应用于藻细胞损伤过程及相关机理研究,这将为发展新的高效灭藻技术及其安全评价方法提供实验基础和依据。
Harmful cyanobacterial blooms are now considered to be a common global environmental problem,and the frequency and intensity of cyanobacterial harmful algal blooms are so increasing that they have posed an imminent threat to drinking water sources. In this work,we reported our recent progress in dealing with the toxic and harmful Microcystic aeruginosa using dielectric barrier discharge（ DBD） non-thermal plasma. We investigated the processes of both algal cellular damage and microcystin-LR（ MC-LR） degradation caused by DBD treatment. The excitation-emission matrix（ EEM） fluorescence spectroscopy combined with fluorescence regional integration technique（ FRI） was employed to monitor the dynamic changes of algal cell inclusion substances including phycocyanin,chlorophyll and metabolites during the treatment,which provided the quantitative evaluation as also checked and confirmed by high performance liquid chromatography（ HPLC）. The results showed that algal cellular inclusions were first released into the extracellular environment,and then degraded gradually during the DBD treatment; in particular,the cyanobacterial toxin MC-LR released from algae cells during DBD treatment could eventually be completely degraded and removed. This work therefore not only demonstrates that nonthermal DBD plasma can effectively inactivate algae cells with simultaneous removal of microcystins,but also suggests that EEMs as a rapid real-time analysis method can be very useful for revealing the plasma-induced processes of algae cell damage and microcystin degradation as well as their underlying mechanisms.
出处
《生物学杂志》
CAS
CSCD
2017年第2期21-25,共5页
Journal of Biology
基金
国家自然科学基金(No.11635013
No.11475217
No.21207137)
关键词
介质阻挡放电(DBD)
低温等离子体
三维荧光光谱
铜绿微囊藻
藻毒素
氧化和降解
dielectric barrier discharge（DBD）
nonthermal plasma
excitation-emission matrix fluorescence spectroscopy
microcystic aeruginosa
microcystin
oxidation and degradation
