摘要
目的探讨阴茎海绵体内损伤结合丝素蛋白膜神经修复神经生长因子的疗效。方法将36只雄性健康SD大鼠随机分成三组,海绵体神经切断组、丝素蛋白膜联合神经生长因子组及丝素蛋白膜修复组。每组12只。海绵体神经切断组大鼠单纯切断双侧的海绵体神经,不做其他处理;对丝素蛋白膜联合神经生长因子F组大鼠先制作海绵体神经缺损的模型,然后用丝素蛋白膜桥接进行修复,局部注入神经生长因子30μl;丝素蛋白膜修复组仅用丝素蛋白膜对缺损的海绵体神经进行桥接修复;术后90天对三组大鼠进行:阿朴吗啡试验,计数SD大鼠阴茎勃起次数、勃起率。应用免疫组化方法检测大鼠阴茎中后部海绵体组织中神经型一氧化氮合酶的表达。应用SPSS17.0软件,所获数据采用方差分析、t检验和X^2检验。结果阴茎勃起率A组与B组、A组与C组比较,P均<0.005;B组与C组比较,P>0.05。勃起次数A组与B组、A组与C组比较,P均<0.0005;B组与C组比较,P<0.001。对缺损的海绵体神经断端进行桥接修复后将自制的神经生长因子90天后NOS神经纤维染色最为明显。阳性纤维数目A组与B组、A组与C组、B组与C组数目比较,P均<0.0005。结论丝素蛋白膜联合神经生长因子对海绵体神经修复与再生具有明显的促进作用,为海绵体神经缺损的临床治疗提供了新的方向及实验依据。
Objective To discuss Inside the penis sponge body damage combined with silk fibroin membrane the curative effect of nerve repair nerve growth factor. Methods 36 healthy male SD rats were randomly divided into three groups, cavernous nerve cut group, the silk fibroin membrane joint nerve growth factor and silk fibroin membrane repair group. 12 in each group. Cavernous nerve to cut off the set of simple cut bilateral cavernous nerve of rats, don＇t do other processing. Of silk fibroin membrane joint nerve growth factor group F rat cavernous nerve defect model, first and then use silk fibroin membrane bridge repair, 30 μ L local injection of nerve growth factor. Silk fibroin membrane repair group only with silk fibroin membrane for bridge repair the defect of the cavernous nerve. After 90 days on three groups of rats： apomorphine test, counting rate of SD rats penile erectile times, erectile. In the application of immunohistochemical method to detect the rat penis sponge tissue at the back of the nerves in the expression of nitric oxide synthase. The obtained data analyzed by variance, t test and χ2test with the SPSS17.0 software. Results The rate of penile erectile group A and group B,group A compared with group C, P〈0.005. Group B compared with group C, P〉0.05. Erectile times in group A and group B, group A compared with group C, P〈0.0005. Group B compared with group C, P〈0.001. To bridge the defect cavernous nerve end after repair will be homemade nerve growth factor 90 days NOS nerve fiber dyeing is most obvious. Number of positive fibers in group A and group B, group A and group C, group B compared with group C number, P〈0.0005. Con-clusion Silk fibroin membrane joint nerve growth factor of cavernous nerve repair and regeneration has an obvious role in promoting, for cavernous nerve defects and provides a new direction and experimental basis for clinical treatment.
出处
《菏泽医学专科学校学报》
2017年第1期1-3,50,共4页
Journal of Heze Medical College
