摘要
瓜氨酸化是指精氨酸在钙离子存在的情况下,由肽酰基精氨酸脱亚胺酶介导引起的一种翻译后修饰。蛋白质瓜氨酸化修饰的上调参与体内多种疾病的发病机制,并具有重要的病理生理学意义。组织细胞中瓜氨酸化位点的鉴定依然迫切需要多种具有更高敏感性和特异性的定量和高通量的检测方法,随着化学衍生技术和质谱技术的发展,新的快速精准的蛋白质组学技术依然具有较好的发展和应用前景。文章主要通过介绍显色剂分析、免疫识别、质谱分析等多种瓜氨酸化多肽的检测方法,以期为复杂生物样本中瓜氨酸位点的鉴定提供新的思路和方向。
Citrullination is a post-translational modification of arginine caused by peptidyl arginine deiminase in the presence of calcium ions. Up-regulation of citrullination plays a significant role in the progress of numerous disorders, and it has important physio- logic and pathological significance. High-throughput and quantitative methods with high specificity and sensitivity are still needed to i- dentify the citrullinated sites in tissue cells. With the development of chemical derivatization and mass spectrometry, new rapid and ac- curate proteomics technology still has a good prospect of development and application. This article aimed to provide new ideas and direc- tions to identify the citrullinated sites in the complex biological samples by introducing strategies available to identify citrullinated pep- tides, such as color development reagent analysis, immunodetection and mass spectrometry.
出处
《医学研究生学报》
CAS
北大核心
2017年第4期421-426,共6页
Journal of Medical Postgraduates
基金
国家自然科学基金(81071419)
江苏省科技支撑计划-社会发展项目(BE2011786)
关键词
瓜氨酸
质谱
类风湿关节炎
Citrullination
Mass spectrometry
Rheumatoid arthritis
