摘要
目的:建立一种高灵敏度、高精密度的多元素快速同时分析的方法,测定长白山药用植物乌苏里瓦韦中10种人体必需微量元素的含量,填补该药用植物微量元素研究的空白,为其质量控制及进一步研究提供科学依据。方法:取3批吉林产乌苏里瓦韦,采用HNO_3-H_2O_2体系将植物全草粉末(过200目筛)湿法消解后,电感耦合等离子体质谱(ICP-MS)法测定其微量元素,以In,Rh为内标,标准曲线法计算各元素的含量。结果:Co元素的检出限为9×10^(-4)μg·g^(-1),其余9种元素的检出限均>1×10^(-3)μg·g^(-1)。10种微量元素的线性范围均为0.05~150μg·g^(-1)。各标准曲线的相关系数均>0.999,RSD均<3.8%,加样回收率为94.8%~105.6%。测得Mn,Zn,Cr,Ni,Cu,V,Mo,Se,Co,Sn各元素质量分数分别为213.7,48.63,13.89,6.543,6.502,2.571,1.231,0.581 9,0.598 2,0.295 7μg·g^(-1)。结论:该快速测定方法灵敏度高、线性范围宽、准确度良好。乌苏里瓦韦中含有较丰富的Mn,Zn,Cr,Ni,Cu,V等人体必需微量元素,在参与多种体内过程中会对该植物的药效发挥会产生直接或间接的影响,为进一步研究乌苏里瓦韦中各元素形态,建立其质量控制标准和活性筛选提供了参考。
Objective： To establish a highly sensitive and highly accurate method for simultaneous and fast multi-elements analysis,determinate the contents of 10 kinds of trace elements in Lepisorus ussuriensis which are essential to human by using this method,fill the blank of studying on the trace elements of this plant,and provide scientific basis for its quality control and further research. Method： Three batches of L. ussuriensis produced in Jilin Province were used as samples. The contents of trace elements were determined by inductively coupled plasma mass spectrometry（ ICP-MS） after the samples were diluted with wet digestion in HNO_3-H_2O_2 system; then with In,Rh as the internal standard,the contents of elements were calculated with standard curve method. Result： For all of the analyzed elements,the standard curve correlation coefficients were all over99. 98%. RSDs were less than 3. 8% and the recovery rates were between 94. 8%-105. 6%. The detection limit was 9 × 10^- 4μg·g^-1for Co element,and were more than 1 × 10^- 3μg·g^-1for other 9 elements. The linear range was0. 05-150 μg·g^-1for all the 10 trace elements,and the contents of elements Mn,Zn,Cr,Ni,Cu,V,Mo,Se,Co,Sn were 213. 7,48. 63,13. 89,6. 543,6. 502,2. 571,1. 231,0. 581 9,0. 598 2,and 0. 295 7 μg·g^-1respectively. Conclusion： The established rapid determination method has high sensitivity,wide linear range and good accuracy. L. ussuriensis has rich contents of Mn,Zn,Cr,Ni,Cu,V and other trace elements essential to human,and they may play direct or indirect effects on the efficacy of this plant when they participate in vivo process. This paper could provide some references for further research on elemental speciation,quality standard establishment and activity screening of L. ussuriensis.
出处
《中国实验方剂学杂志》
CAS
CSCD
北大核心
2017年第9期57-61,共5页
Chinese Journal of Experimental Traditional Medical Formulae
基金
吉林省科技创新人才培育计划-吉林省呼吸道疾病转化医学创新团队项目(20150519015JH)
