摘要
目的:威灵仙作为临床常用的中药,具有祛风除湿、通络止痛、消骨哽的功效,常用于治疗风湿痹痛,为风湿痹痛要药,亦用于治疗诸骨哽咽,同时具有一定的副作用。近年来发现威灵仙提取物对白血病细胞增殖具有抑制作用,该文旨在研究其化学成分,揭示其治疗疾病的物质基础。方法:将威灵仙药材粉碎后,采用乙醇回流提取,得到粗提物,将其分散于水中后,依次使用石油醚、乙酸乙酯、正丁醇萃取;利用D101大孔树脂,硅胶,LH-20羟丙基葡聚糖凝胶,MCI小孔树脂凝胶,RP-18柱色谱对各提取部位进行分离,并通过~1H-NMR,^(13)C-NMR,MS等波谱技术鉴定其结构。结果:从威灵仙药材中分离得到9个化合物,分别鉴定为升麻醇(1),升麻醇-3-O-β-D-木糖苷(2),cimigoside(3),木栓酮(4),(+)-3,3'-bisdemethylpinoresinol(5),5-O-feruloyl-2-deoxy-D-ribono-γ-lactone(6),阿魏酸(7),丁香酸(8),β-谷甾醇(9)。结论:化合物1~6均为首次从威灵仙中分到,其中化合物1~3为首次从铁线莲属中分离得到环菠萝蜜型三萜类化合物。分离得到的化合物进一步丰富了威灵仙的化学成分结构类型,为威灵仙的植物分类、质量控制和药理活性研究提供了新的依据。
Objective： Clematidis Radix et Rhizoma from Ranunculaceae plant is a common traditional Chinese medicine in clinical practice. It has efficacies of eliminating wind and dampness,dredging collaterals to stop pain,and eliminating bone mineral,and can be used to treat many diseases,such as rheumatism and pharyngitis. Meanwhile,it was reported to have certain side effects. In recent years,some findings showed that its extract has an inhibitory effect on the proliferation of leukemia cells. This paper aims to study the chemical constituents from Clematidis Radix et Rhizoma and reveal its material base. Method： Clematidis Radix et Rhizoma were crushed into powder and extracted with ethanol. This residues were dispersed in H_2O,and then extracted successively with petroleum ester, ethyl acetate and butanol. The compounds were isolated by column chromatography on D101 Macroporous resin,silica gel,Sephadex LH-20,MCI,and RP-18,and the structures were identified through spectroscopic methods,including ~1H-NMR,^（13）C-NMR and MS. Result： Compounds 1-9from this plant were identified as cimigenol（ 1）,cimigenol-3-O-β-D-xylpyranoside（ 2）,cimigoside（ 3）,friedelin（ 4）,（ ＋）-3,3＇-bisdemethylpinoresinol（ 5）,5-O-feruloyl-2-deoxy-D-ribono-γ-lactone（ 6）,ferulic acid（ 7）,syringic acid（ 8） and β-sitosterol（ 9）. Conclusion： Compounds 1-6 were isolated from the rhizoma of Clematidis Radix et Rhizoma for the first time. Compounds 1-3 were cycloartane triterpenoid and obtained from Clematis genus for the first time. The isolated compounds further enrich chemical structure types from Clematidis Radix et Rhizoma,and offer a new basis to study plant taxonomy,quality control and pharmacological function.
出处
《中国实验方剂学杂志》
CAS
CSCD
北大核心
2017年第9期41-45,共5页
Chinese Journal of Experimental Traditional Medical Formulae
基金
国家自然科学基金项目(81460648)
关键词
威灵仙
铁线莲属
化学成分
环菠萝蜜型三萜类
Clematidis Radix et Rhizoma
Clematis
chemical constituent
cycloartane triterpenoid
