摘要
将多级液液微萃取与气相色谱-质谱联用技术相结合建立了白酒中微量角鲨烯的快速定量方法。将白酒稀释后,用戊烷-乙醚混合溶剂(1∶3,体积比)进行多级微萃取,有机相浓缩后气质联用分析。结果显示,角鲨烯在0.62~159.88μg/L范围内具有良好的线性关系,相关系数为0.995 5,角鲨烯的检出限为0.17μg/L,当加标浓度为2,20,40μg/L时回收率分别为78.1%,85.0%和103.5%,相对标准偏差(RSD)分别为12.1%,8.9%和7.0%。该方法具有简便快速、准确灵敏、萃取效率高、有机溶剂消耗少等优点,适合白酒中痕量角鲨烯的定量分析。
A multistage liquid-liquid microextraction（LLME） coupled with GC-MS method was developed to determine squalene in Chinese liquor.After diluting the liquor sample,a multistage microextraction was carried out using pentane-diethyl ether mixture（1∶3,by volume）,then the organic phase was concentrated with nitrogen,and analyzed by GC-MS.The result indicated that squalene had a good linearity in the range of 0.62-159.88 μg/L,with a correlation coefficient of 0.995 5.The detection limit of squalene was as low as 0.17 μg/L.The average recoveries of squalene at spiked levels of 2,20,40 μg/L were 78.1%,85.0%,103.5%,respectively,with relative deviations of 12.1%,8.9%,7.0%,respectively.With the advantages of simplicity,accuracy,high-extraction efficiency and low consumption of organic solvent,this method was suitable for the quantitative analysis of squalene in Chinese liquor.
出处
《分析测试学报》
CAS
CSCD
北大核心
2017年第4期534-538,共5页
Journal of Instrumental Analysis
