摘要
以广西南亚热带农业科学研究所自育甘蔗新品系桂南亚08-212的幼嫩叶片作为外植体,采用不同2,4-D浓度对幼嫩叶片的切片进行愈伤诱导,以不同6-BA浓度对甘蔗愈伤进行分化诱导和增殖培养,以不同NAA浓度诱导甘蔗组培苗生根。结果表明:适宜甘蔗幼嫩叶片愈伤诱导的培养基为改良MS+2,4-D 2.0 mg/L,诱导分化培养以改良MS+6-BA 2.0 mg/L为宜,增殖培养以改良MS+6-BA 2.0 mg/L为宜,适宜的生根培养基为改良MS+NAA 5.0 mg/L。
The tender leaves of sugarcane GNY 08-212 as explant, the experiment was carried out by using different concentration of 2,4 D for slices of tender leaf callus induction, different concentration of 6-BA for callus induction and multiplication culture, and different concentration of NAA for induction rooting of sugarcane somaclone. The results show that： the effect of the culture MS ＋ 2,4 D 2.0 mg/L for tender leaves callus induction displayed the best; the effect of the culture MS ＋ 6-BA 2.0 mg/L for differentiation culture displayed the best;the effect of the culture MS ＋ 6-BA 2.0 mg/L for multiplication culture was the best; the effect of the culture MS＋ NAA 5.0 mg/L for rooting medium displayed the best.
出处
《中国糖料》
2017年第3期9-10,共2页
Sugar Crops of China
基金
基金项目:高产
高糖
抗逆性强甘蔗优良新品种选育(桂科攻1598006-1-1E)
关键词
甘蔗
桂南亚08-212
组培快繁
sugarcane
GNY08-212
rapid propagation technology of tissue culture
