摘要
目的 研究双酚A（BPA）对离体培养青春前期大鼠睾丸支持细胞N-钙黏蛋白（N-cadherin）、波形蛋白（Vimentin）和卵泡刺激素受体（FSHR）表达的影响.方法 取3只健康18-21日龄SPF级SD雄性大鼠进行睾丸支持细胞原代培养,48 h后分别暴露于含终浓度为0（溶剂对照）、30、50、70μmol/L BPA的培养基中染毒24 h.采用MTT法检测支持细胞的细胞活性,分别采用实时荧光定量PCR（real-time quantitative PCR）法和Western blotting法检测支持细胞N-cadherin、Vimentin及FSHR mRNA和蛋白的表达水平.结果 与溶剂对照组相比,50、70μmol/L BPA染毒组睾丸支持细胞的存活率均下降,差异有统计学意义（P〈0.05）;且随着BPA染毒剂量的升高,睾丸支持细胞的存活率呈逐渐下降的趋势.与溶剂对照组相比,各剂量BPA染毒组睾丸支持细胞FSHR mRNA的表达水平均较高,而Vimentin mRNA的表达水平均较低,仅30μmol/L BPA染毒组睾丸支持细胞N-cadherin mRNA的表达水平升高,差异均有统计学意义（P〈0.05）.与溶剂对照组比较,各剂量BPA染毒组睾丸支持细胞Vimentin蛋白及50、70μmol/L BPA染毒组睾丸支持细胞FSHR蛋白的表达水平均较低,仅50μmol/L BPA染毒组睾丸支持细胞N-cadherin蛋白的表达水平升高,差异均有统计学意义（P〈0.05）.结论 BPA通过干扰支持细胞内N-cadherin、Vimentin和FSHR蛋白的表达来影响正常的精子发生,从而产生睾丸毒性.
Objective To explore the effects of BPA on the expression of N-cadherin, Vimentin and FSHR in rat Sertoli cells. Methods Primary Sertoli cells collected from prepuberty rats （18-21 d） were cultured for 48 h, and then they were treated with 0, 30, 50, 70μmol/L BPA respectively for 24 h. The methods of MTT, real-time quantitative PCR and Western blotting were utilized to measure the cell ability of Sertoli cells, the mRNA and protein expression levels of N-cadherin, Vimentin and FSHR respectively. Results Compared with control, the cell abilities of Sertoli cells in 50 μmol/L BPA group and 70 μmol/L BPA group increased significantly （P〈0.05）. The cell abilities of Sertoli cells decreased with the increases of exposure doses of BPA. Compared with control,the expression of N-cadherin mRNA only increased in 30μmol/L BPA group （P〈0.05）, the expression of Vimentin mRNA decreased significantly in all doses group of BPA （P〈0.05）, the expression of FSHR mRNA increased in all doses group of BPA （P〈0.05）. Compared with the control, the protein levels of N-cadherin increased significantly in 50 μmol/L BPA group （P〈0.05）, the protein levels of Vimentin decreased significantly in all doses group of BPA（P〈0.05）, the protein levels of FSHR decreased significantly in 50μmol/L BPA group and 70μmol/L BPA group （P〈0.05）. Conclusion The mechanism of testicular toxicity from BPA might be the alterations of N-cadherin,Vimentin and FSHR by disturbing normal spermatogenesis.
出处
《中华劳动卫生职业病杂志》
CAS
CSCD
2017年第2期101-105,共5页
Chinese Journal of Industrial Hygiene and Occupational Diseases
基金
国家自然科学基金（81172623）
