摘要
目的:建立蜈蚣药材的定性定量分析方法。方法:采用薄层色谱法对蜈蚣药材进行定性鉴别,以三氯甲烷-甲醇-甲酸(10∶1∶1)为展开剂,在紫外光灯(254 nm)下检视;运用高效液相色谱法测定3,8-二羟基喹啉的含量,采用Kromasil C_(18)(250 mm×4.6 mm,5μm)色谱柱,流动相为甲醇-10 mmol·L^(-1)的磷酸二氢钾溶液(32∶68),流速0.8 m L·min^(-1),检测波长252 nm,柱温40℃。结果:薄层鉴别斑点清晰,分离度良好;含量测定中,3,8-二羟基喹啉的线性范围为0.036 2~0.289 6μg(r=0.999 4),平均加样回收率为99.2%(RSD=1.4%,n=6),17批样品中3,8-二羟基喹啉的含量范围为0.175~2.28 mg·g^(-1)。结论:本文建立的薄层色谱鉴别及高效液相色谱含量测定方法可用于蜈蚣药材的质量控制。
Objective： To establish qualitative and quantitative methods of Scolopendra. Methods： TLC method was used for qualitative identification of Scolopendra. The plate was developed with chloroform-methanol- formic acid （ 10 ： 1 ： 1 ）and detected under ultraviolet light at 254 nm. The content of 3,8-dihy droxy quinolone was determined by HPLC. A Kromasil C18 （250 mm × 4.6 mm, 5 μm ）column was used, and the mobile phase was methanol-10 mmol· L^-1 potassium dihydrogen phosphate （ 32 ： 68 ）at a flow rate of 0.8 mL· min^-1. The detection wavelength was set at 252 nm and the column temperature was 40 ℃. Results： TLC spots were clear and well separated. 3, 8-dihydroxy quinoline showed a good linear relationship at the range of 0.036 2-0.289 6 μg （ r=0.999 4 ）. The average recovery was 99.2% （ RSD=1.4%, n=6 ） and the content range in seventeen batches of samples was 0.175- 2.28 mg· g^-1. Conclusion： The proposed TLC identification and HPLC assay methods could be used for quality control of Scolopendra.
作者
刘武占
范建伟
李艳芳
苏瑞强
张贵民
LIU Wu-zhan FAN Jian-wei LI Yan-fang SU Rui-qiang ZHANG Gui-min(Lunan Pharmaceutical Group Co., Ltd., State Key Laboratory of Generic Manufacture Technology of Chinese Traditional Medicine, Shandong Provincial Key Laboratory of New Manufacture Technology of Chinese Traditional Medicine, Linyi 276006, Chin)
出处
《药物分析杂志》
CAS
CSCD
北大核心
2017年第4期639-643,共5页
Chinese Journal of Pharmaceutical Analysis
基金
山东省自主创新及成果转化专项(创新型产业集群)计划(2015ZDJQ05003)
