摘要
初步建立了寨卡病毒(Zika virus)IgG抗体的间接ELISA检测方法,为开发相应的血清学诊断试剂提供理论依据。选取寨卡病毒四种抗原,即E蛋白胞外区(Ectodomain)、NS1蛋白、C蛋白和rEⅢ蛋白进行重组表达和纯化,分别包被ELISA板并用间接法检测寨卡病人临床血清和正常人血清,确定每种检测抗原的检测敏感度和特异度。重组表达并纯化的四种寨卡病毒抗原浓度和纯度均达到检测要求。间接ELISA检测结果显示E蛋白胞外区和NS1蛋白的检测敏感度和特异度均达到较高水平,但C蛋白和rEⅢ蛋白的检测敏感度很低,不适合作为寨卡病毒的检测抗原。本研究初步评价了寨卡病毒四种抗原检测相应IgG抗体的敏感度和特异度,为研制血清学诊断试剂奠定了基础。
An indirect enzyme-linked immunoassay（ELISA）-based serological method for detection of IgG antibodies to the Zika virus was established.Four types of Zika-virus antigen（ectodomain of E protein;NS1protein;rEIII protein;C protein）were expressed,purified and coated on ELISA plates.Indirect ELISA methods were used for the sera of Zika-infected patients and those of healthy subjects to determine the sensitivity and specificity of each type of coated antigen.The concentration and purity of four Zika antigens met the criteria for detection.The results of indirect ELISA showed that the sensitivity and specificity of detection for anti-E（ectodomain）and anti-NS1 IgG antibodies were high.However,the sensitivity of detection for anti-rEIII and anti-C antibodies was very low,so this method was not suitable for detection of virus antibodies.By evaluating the sensitivity and specificity of detection for specific IgG antibodies of four Zika antigens,we have laid the foundation for the development of diagnostic agents.
出处
《病毒学报》
CAS
CSCD
北大核心
2017年第2期253-257,共5页
Chinese Journal of Virology
基金
科技部改革发展专项--寨卡疫情防控科技攻关应急专项(项目号:国科发资【2016】273号)
题目:寨卡病毒快速检测试剂与装备的研发
