摘要
[目的]分别采用高效液相-荧光检测器、紫外可见分光光度仪对混合制剂中的草铵膦、草甘膦2种组分进行检测。[方法]草铵膦检测:以邻苯二甲醛为衍生剂,甲醇-0.05 mol/L磷酸二氢钾(体积比35:65)为流动相,以C_(18)为色谱柱,激发波长295 nm,发射波长450 nm,试样衍生后进行高效液相-荧光检测器检测。草甘膦检测:试样亚硝化反应后采用紫外可见分光光度仪检测,检测波长为242 nm。[结果]草铵膦质量浓度在30~70 mg/L范围内线性关系良好,相关系数为0.9957,检测限为0.53 mg/L。草甘膦质量浓度在5~25 mg/L范围内线性关系良好,相关系数为0.9986,检测限为0.02 mg/L。[结论]该检测方法反应条件温和,灵敏度较高,重现性好,适用于草铵膦·草甘膦混合制剂中草铵膦、草甘膦的测定。
[Aims] Determination of mixture formulation of glufosinate and glyphosate by high-performance liquid chromatography （HPLC） with fluorescence detector and uv-vis spectrophotometer was studied. [Methods] Determination of glufosinate was conducted with o-phthalaldehyde as the derivating agent, the mixture of methanol and 0.05 mol/L of potassium dihydrogen phosphate （35：65, by vol） as mobile phase, C18 chromatographic column as stationary phase at excitation wavelength of 295 mn and emission wavelength of 450 nm by HPLC with fluorescence detector after derivative. Determination of glyphosate was conducted by uv-visible spectrophotometer at 242 nm after the nitrosation reaction. [Results] When the concentration of glufosinate was 30-70 mg/L, the linear correlation coefficient was 0.9957, and the limit of detection was 0.53 mg/L. When the concentration of glyphosate was 5-25 mg/L, the linear correlation coefficient was 0.9986, and the limit of detection was 0.02 mg/L. [Conclusions] The method has the advantages of simple, high sensitivity and reproducibility, which is suitable for the analysis of mixture formulation of glufosinate and glyphosate.
作者
余青云
穆允玲
韦少平
黄科润
韦茂春
柯敏
YU Qing-yun MU Yun-ling WEI Shao-ping HUANG Ke-run WEI Mao-chun KE Min(Guangxi Research Institute of Chemical Industry, Narming 530001, China)
出处
《农药》
CAS
CSCD
北大核心
2017年第4期262-264,共3页
Agrochemicals
基金
基金项目:桂科转(1599002-8)
桂科(AB16380125)
桂科(AA16380006)
关键词
草甘膦
草铵膦
柱前衍生
荧光检测
glufosinate
glyphosate
pre-column derivatization
fluorescence detection
