摘要
目的以哮喘患儿为研究对象,观察分析哮喘患儿急性发作期和疾病缓解期血清总IgE、IL-4、IFN-γ、IgG4的临床免疫学指标与哮喘发作的相关性,并探讨哮喘患儿发生的免疫学机制。方法选取30例哮喘患儿为病例组,同时选择30例入托前3~4岁门诊体检儿童为健康对照组。采用免疫散射比浊法检测哮喘患儿急性发作期和缓解期血清总IgE、IgG4的水平,运用酶联免疫吸附实验(ELISA)检测其IL-4和IFN-γ的水平。结果病例组急性期血清总IgE、IL-4、IgG4水平均明显高于缓解期(P<0.05),而IFN-γ水平显著低于缓解期(P<0.05);病例组缓解期血清总IgE、IL-4水平明显高于健康对照组(P<0.05),IFN-γ水平明显低于健康对照组(P<0.05);病例组缓解期IgG4水平和健康对照组比较,差异无统计学意义(P>0.05)。病例组急性发作期IL-4与总IgE呈正相关性(r=0.751 7)。结论检测血清总IgE、IL-4、IFN-γ和IgG4的水平可辅助诊断儿童哮喘;监测血清总IgE、IL-4、IFN-γ、IgG4水平可进行疗效判断。
Objective To investigate the immunological pathogenesis of asthma in the childhood through detecting the levels of total IgE,IL -4, IFN - γ, IgG4 in acute stage and remission stage and analyzing the relevance. Methods 30 children with asthma were collected from pediatric patient as the case group and 30 health children which were 3 -4 years old as control group. The total serum of IgE, IgG4 were detected by immuno - scatter turbidmetry. IL - 4 and IFN - γ were detected by ELISA. Results In acute phase, the serum levels of total IgE, IL - 4, IgG4 were significantly higher than those of the catabasis phase （ P 〈 0.05 ）, while IFN - γ/level was significantly lower than those of the catabasis phase（ P 〈 0.05 ）. The serum leves of total IgE, IL -4 in the catabasis phase was significantly higher than the control（P 〈 0.05）. IFN - γ levels were significantly lower than the control （ P 〈 0.05 ）. The level of IgG4 in catabasis stage was no statistically different （ P 〉 0.05 ）. There were positive correlation between total serum IgE and IL - 4 in acute stage （ r = 0.751 7 ）. Conclusion The serum of total IgE ,IL - 4 ,IgG4 and IFN - γ is helpful to diagnosis the asthma in children. Monitoring IgE, IL - 4, IgG4 and IFN - γ could know the efficacy of asthma in children.
出处
《宁夏医学杂志》
CAS
2017年第4期306-308,共3页
Ningxia Medical Journal
基金
宁夏医科大学校级课题(XM201448)
