摘要
目的探讨黄芪甲甙介导钙敏感受体(CaSR)对缺血缺氧心肌的保护作用及机制。方法将32只SD大鼠随机分为正常对照组、模型组(IS0)、黄芪甲甙低剂量组(ISO+AST IV 10 mg/kg)、黄芪甲甙高剂量组(ISO+AST IV 20 mg/kg);HE染色光镜下观察不同组别心肌组织的病理学改变,检测不同组别血清肌酸激酶及肌酸激酶同工酶含量;Western blotting法检测CaSR蛋白的表达。结果与模型组相比黄芪甲甙低、高剂量组能不同程度减轻心肌病理改变,降低血清肌酸激酶以及肌酸激酶同工酶含量,下调CaSR蛋白表达水平(P<0.05)。结论黄芪甲甙对异丙肾上腺素诱导大鼠缺血缺氧心肌有保护作用;其机制可能为下调CaSR蛋白表达减轻CaSR对心肌损伤的作用。
Objective To explore the protective effects and mechanism of Astragaloside IV mediated calcium sensi- tive receptor （CaSR） on ischemia anoxic myocardial. Methods 32 SD rats were randomly divided into normal control group,model group （IS0） glucoside, astragaloside IV low dose group （ ISO ＋ AST IV 10 mg/kg）, astragaloside IV high dose group （ISO ＋ AST IV 20 mg/kg）.Observing myocardial tissue pathology change of different groups by HE staining, de- tecting CK and CK-MB content; detecting CaSR protein expression by Western Blotting method. Results Compared with model group astragaloside IV low dose group and high dose group can reduce the myocardial pathological varying degrees changes,reduce serum CK and CK-MB,lower CaSR protein expression levels （P〈 0.05）. Conclusion Astragaloside IV can protect isehemia anoxic myocardial injury induced by ISO; The mechanism may reduce the CaSR protein expression and effects on myocardial injury.
作者
邓纯
杨云华
左袁博教
张雅林
蒋德林
DENG ChuM YANG Yunhua ZUO Yuanbojiao et al(Department of Pediatrics, Central Hospital of Yongzhou, Yongzhou 425000, HuMan, Chin)
出处
《中南医学科学杂志》
CAS
2017年第2期140-144,共5页
Medical Science Journal of Central South China
