摘要
目的从分离自海南红树林根际糖多胞菌Saccharopolyspora sp.YIM M13568菌株中分离和鉴定红霉素类抗菌活性产物。方法采用反相硅胶色谱、凝胶色谱和正相硅胶色谱等技术,对菌株YIM M13568发酵产物进行分离纯化;通过1D、2D NMR和HR-ESI-MS进行结构测定。结果鉴定了3个红霉素家族抗生素的结构,包括anhydroerythromycin A(1)、3-O-mycarosylerythronolide B(2)和一个新的红霉素类化合物命名为sporeamicin D(3),并测试了它们的抗菌活性。结论糖多胞菌YIM M13568菌株是红霉素类抗生素的新资源微生物。
Objective Mangrove is rich in bioactive natural products. As a continuation of our search for bioactive small molecules from mangrove actinomycetes, this study isolated and identified antibacterial active erythromycin analogues from Saccharopolyspora sp. YIM M13568 which was isolated from a mangrove rhizosphere soil sample collected in Wenchang County, Hainan Province. Methods The strain Saccharopolyspora sp. YIM M13568 was cultured for 14d on MP agar media （mannitol 2%, peptone 2%, sea salt 2.5%, agar 1.5%, pH7.2-7.5） plates at a total volume of 12L at 38℃. The fermented cultures were diced and extracted three times overnight with EtOAc-MeOH-AcOH （80：15：5, V/V/V） at room temperature. After the removal of solvents under vacuum, the crude extract was partitioned between H20 and EtOAc. The EtOAc-soluble partition was dissolved in 80mL of 95% methanol and extracted five times with an equal volume of petroleum ether to afford the defatted MeOH extract （4.0g）. The extracts were purified with various chromatographic methods, including column chromatography over Sephadex LH-20 and normal silica gel, MPLC and HPLC to afford three pure components. The structures of all pure components were elucidated on the basis of the analysis of 1D, 2D NMR, and HR-ESI-MS spectroscopic data. The antimicrobial activities were measured by paper disc diffusion and double dilution methods on Candida albicans 5314, Staphylococcus aureus ATCC25923, Bacillus subtilis ATCC6051, Pseudomonas aeruginosa PA01 and Salmonella enterica serovar Typhimurium UK-1 Z8956. Results Three erythromycin derivatives were obtained, and determined to be anhydroerythromycin A（1）, 3-O-mycarosylerythronolide B（2） and a new one named sporeamicin D（3）, respectively. At the loading amount of 30μg/disc, compounds 27 and 3 were found to have no antimicrobial activities. Compound 1 showed evident inhibitory activities on S. aureus ATCC25923 and B. subtilis ATCC6051 with MICs of 1.6μmol/L and 6.4μmol/L as compared to the MICs of 0.8μmol/L a
作者
孙明伟
丁艳娇
鲁春华
沈月毛
Sun Ming-wei Ding Yan-jiao Lu Chun-hua Shen Yue-mao(Key Laboratory of Chemical Biology （Ministry of Education）, School of Pharmaceutical Sciences, Shandong University, Ji＇nan 250012)
出处
《中国抗生素杂志》
CAS
CSCD
北大核心
2017年第4期J0026-J0030,共5页
Chinese Journal of Antibiotics
基金
国家自然科学基金面上项目(No.81673317)
