摘要
目的评价负载万古霉素明胶（gelatin）／β-磷酸三钙（β—tricalciumphosphate，β-TCP）复合多孔材料的缓释性能，验证其在感染性骨缺损动物模型治疗中的感染清除及骨缺损修复性能。方法分别制备不同β-TCP含量（质量分数为0、10％、30％和50％）的明胶／β-TCP载万古霉素复合多孔材料。对其进行扫描电镜观察、孔隙率及力学强度测试。采用K—B法检测负载万古霉素明胶／β-TCP复合多孔材料体内的缓释性能。制备兔胫骨慢性骨髓炎模型，清创后分为四组进行修复，分别为空白对照组、G-0TCP组、G-10TCP组及G。30TCP组。术后4、8周摄X线片并取材行HE染色观察感染清除及骨修复情况。结果β-TCP颗粒的加入对孔径无明显影响，但可增加孔与孔间的连通性。随着β-TCP颗粒含量的增加，孔隙率明显增大，但当β-TCP颗粒含量达到质量分数50％时孔隙率下降。复合多孔材料压缩模量随着β-TCP颗粒的加入而逐渐增大，各组间的差异有统计学意义。体内缓释实验结果显示G-0TCP材料可持续释放万古霉素8周；随着β-TCP含量的增多，缓释时间逐渐缩短；G-50TCP材料仅能持续释放万古霉素3周。动物实验中植入载药复合材料8周后，空白对照组感染未清除，大量死骨形成；G-0TCP组及G-10TCP组感染清除，但未能修复骨缺损；G-30TCP组清除感染并修复骨缺损。结论明胶／β-TCP复合多孔材料对万古霉素具有良好的缓释效果；质量分数30％β-TCP的复合多孔材料具有最佳的孔径、孔隙率、力学强度及体内缓释效果，并在动物实验中表现出最佳的感染清除及骨缺损修复能力。
Objective To confirm the vancomycin loaded gelatin/β-TCP composite porous scaffolds could be used as sustained-release system, and investigate its efficiency of eliminating infections and repairing bone defects for the treatment of infected bone defects in rabbit. Methods The biodegradable gelatin sponge containing different contents （0, 10%, 30%, 50%） of β- tricalcium phosphate ceramic （β-TCP） was prepared for the controlled-release of vancomycin and labeled with G-0 TCP, G-10 TCP, G-30 TCP and G-50 TCP respectively. Examinations of scanning electron microscopy, porosity analyses and mechanical test were performed. The K-B method was used to investigate the controlled release of vancomycin. Chronic Methicillin-resistant Staph- ylococcus aureus osteomyelitis models of rabbit were established. After thorough debridement, the infected bone defects were treat- ed in four different groups： blank control group, G-0 TCP group, G-10 TCP group, and G-30 TCP group. At 4 and 8 weeks after implantation, X-ray and histological examinations were carried out to investigate the efficiency of eliminating infections and repairing bone defects. Results The prepared gelatin/β-TCP scaffold exhibited a homogeneously interconnected-3-D porous structure. And the β-TCP granules were localized evenly on the walls of the composite scaffold. There were no significant differences in the pore size of different scaffolds. However, the β-TCP granules can improve the interconnection. The porosity exhibited an obvious increase in G-10 TCP and G-30 TCP composite scaffolds compared with G-0 TCP scaffold. In contrast, too high content of β-TCP granules decreased the porosity. And the porosity exhibited an obvious reduction in the G-50 TCP composite scaffold. The compressive modulus of the vancomycin loaded scaffolds increased with the increase of the β-TCP amount. The scaffold G-0 TCP ex- hibited the longest duration of vancomycin release and the duration reached 8 weeks. With the increased content of β-TCP gran- ules, the releas
出处
《中华骨科杂志》
CAS
CSCD
北大核心
2017年第8期498-507,共10页
Chinese Journal of Orthopaedics
基金
国家自然科学基金（81301577）
上海市青年科技启明星计划（15QA1401000）
高等学校博士学科点专项科研基金（20130071120062）
关键词
明胶
磷酸钙类
万古霉素
迟效制剂
骨髓炎
Gelatin
Calcium phosphates
Vancomycin
Delayed-action preparations
Osteomyelitis
