摘要
目的:探讨小分子RNA(microRNA)miR-363-3p和miR-5100在非小细胞肺癌中的表达及其临床意义。方法:利用荧光定量PCR的方法,检测肿瘤组织、癌旁组织及远离肿瘤的正常组织中致癌基因Myc的mRNA和miR-363-3p、miR-5100的表达,然后分析miR-363-3p和miR-5100与临床病理特征之间的相关性。结果:Myc在肿瘤组织中表达明显升高;miR-363-3 p在肺癌组织中的表达明显低于正常组织(P<0.001),而在癌旁组织中的表达却远远高于正常组织(P<0.05);miR-5100在肺癌组织中的表达显著地高于正常组织(P<0.001),并且癌旁组织中的表达也高于正常组织(P<0.05)。临床相关性分析显示,miR-363-3p的表达与淋巴结转移呈正相关(P<0.001),miR-5100的表达与临床分期也呈正相关性(P<0.05)。结论:miR-363-3p和miR-5100可能参与了非小细胞肺癌早期的发生和转移,并可能作为早期诊断和预后的分子标志物。
ObjectiveTo detect the expression of miR -363 - 3 p and miR -5100 in non - small cell lung canc-er. Methods：We performed the quantitative polymerase chain reaction（ qPCR） to detect the expression of Myc,miR -363 -3 p and miR -5100 in tumor tissue,adjacent normal lung tissue and paratumor. The association between the differentially expressed miRNAs and the clinicopathological features of the associated tumors,was analyzed. Results： Myc was upregulated in tumor from NSCLC cases（ P 〈 0. 01）. miR - 363 - 3p was examined for significant low - ex-pression in tumor tissue（P 〈0. 001） ,while miR -5100 was upregulated in tumor tissue. The associations between the miRNA and their clinicopathological features were also investigated. miR -363 - 3p was associated with lymph node status（P 〈0. 001） and miR -5100 was associated with pathologic stages（P 〈 0. 05） . Conclusion：The expression of deregulated miR -363 -3 p and miR -5100 may participate the development of non - small cell lung cancer.
出处
《现代肿瘤医学》
CAS
2017年第10期1560-1563,共4页
Journal of Modern Oncology
基金
国家自然科学基金(编号:81570062)
广东医科大学科研项目(编号:M2014032)
