摘要
目的探讨血必净干预对水下爆炸致兔急性肺损伤肿瘤坏死因子(tumor necrosis factor-alpha,TNF-α)、中性粒细胞弹性蛋白酶(neutrophil elastase,NE)在血清和支气管肺泡灌洗液(broncho alveoloar lavage fluid,BALF)中表达的影响。方法将20只实验兔随机分为爆炸损伤组和血必净治疗组.以水下爆炸装置对实验免造成急性肺损伤,爆炸后24 h测定血清和BALF中的TNF-α和NE含量。结果血必净治疗组血清(353.30±166.86 ng/L)TNF-α浓度与爆炸损伤组(552.30±169.64 ng/L)相比显著下降(t=2.645,P=0.016)。血必净治疗组NE在血清中的表达(63.40±36.09 ng/ml)显著低于爆炸损伤组(97.60±36.20 ng/ml;t=2.116,P=0.049);在BALF的表达(102.10±9.50 ng/ml)也低于爆炸损伤组(136.70±13.60 ng/ml;t=6.593,P=0.000)。结论血必净干预治疗能够显著降低水下爆炸致兔急性肺损伤血清TNF-α水平以及NE在血清和BALF中的浓度。
Objective The concentration of NE and TNF-α was detected for elucidating the change of them in sera and bron- choalveolar lavage fluid （BALF） of rabbits with acute lung injury interposed by XueBiJing therapy in underwater explosion. Methods Underwater explosion decive was applied to cause acute lung injury of rabbits. The 20 rabbits were randomly di- vided into two groups which were injury group and XueBiJing therapy group, respectively. The concentration of NE and TNF-α in sera and BALF were detected by ELISA method in 24 hours after bursting. Results The concentration of TNFa in sera （353.30±166.86 ng/L） of rabbits in therapy group were significantly lower than those in injury group （552. 30± 169.64 ng/L;t=2. 645,P=0. 016）. The concentration of NE in sera （63.40±36.09 ng/ml） were lower than that of rabbits in injury group （97. 60±36.20 ng/ml;t=2. 116,P=0. 049）. At the same time the concentration of NE in BALF （102, 10± 9.50 ng/ml） of rabbits in therapy group were significantly lower than those in injury group （136.70 ±13.60 ng/ml; t= 6. 593,P=0, 000）. Conclusion The expression of TNF-α in sera and NE in sera and BALF in rabbits with acute lung injury interposed by XueBiJing in underwater explosion were lower than those of injury group.
出处
《现代检验医学杂志》
CAS
2017年第2期119-122,共4页
Journal of Modern Laboratory Medicine
基金
南京军区医学重点课题资助项目(14ZD20)
