摘要
目的探究热适应/热射病大鼠模型血清中microRNA let-7b表达的变化及其与HSP70,IL-6,TNF-αTGF-β和IL-12的相关性,分析其临床意义。方法在第二军医大学实验动物中心选取肛温、体重、用龄基本一致的雄鼠60只随机均分为对照组、热适应组、热射病组,于仿真热气候动物舱中建立热适应/热射病大鼠模型后采集心尖血并分离血清。以实时荧光定量PCR(qRT-PCR)法检测血清中microRNA let-7b,以酶联免疫吸附法(ELISA)检测血清中HSP70,IL-6,TNF-α,TGF-β和IL-12的蛋白水平。三组间计量资料的比较采用Kruskal-Wallis H检验,以Spearman秩相关系数表示热射病组两变量之间的相关性。结果 miRNA let-7b在对照组、热适应组、热射病组的M分别为0.99,1.04和1.93,Q分别为0.30,0.25和0.44,差异具有统计学意义(x^2=38.95,P<0.001),组间两两比较结果显示let-7b在对照组与热适应组表达差异无统计学意义(P>0.05),在对照组与热射病组、热适应组与热射病组表达差异具有统计学意义(P<0.05);Spearman相关性分析显示热射病组let-7b/与HSP70,TNF-α和IL-6呈正相关,差异具有统计学意义(r_s=0.579,0.498和0.609,P<0.05)。结论 miRlet-7b可能参与了热射病病理过程,为临床监测高温湿环境下患者的状态提供了潜在的评价指标。
Objective To explore the change of microRNA let-7b in heat acclimatization/heat stroke rat models and its rela- tion with HSP70,IL-6,TNF-α,TGF-β and IL-12,and analyze its clinical significance. Methods 60 male rats with almost the same anal temperature, weight, and weeks＇ age were selected from Laboratory Animal Center in the Second Military Medical University. They were randomly divided into control, heat acclimatization, heat stroke groups averagely. Heat acclimatiza- tion/heat stroke rat models were built in hot climate simulated animal tank,blood was collected from cordisapex and serum was separated. Real-time fluorescent quantitative polymerase chain reaction （qRT-PCR） was used to test microRNA let-7b in serum, enzyme-linked immunosorbent reaction （ELISA） was to measure the protein level of HSP70, IL-6, TNF-α,TGF-β and IL-12 in serum. Kruskal-Wallis H test was used to analyze quantitative data in the three groups,Spearman rank correla- tion coefficient was used to reveal relation between two variables in heat stroke group. Results M values of miRNA let-7b in control, heat acclimatization, heat stroke groups were 0.99,1.04 and 1.93 separately, Q values were 0.30,0.25 and 0.44 （x^2 = 3K 95 ,P〈（0. 001）, separately, with statistical significance, The results Of pairwise comparison showed no statistical differ- ence in control and heat acclimatization （P〈0.05）, but there were differences in controland heat stroke, heat acclimatization and heat stroke groups statistically （P〈0.05）. Spearman correlation analysis showed that in heat stroke group,let-7b was positively related with HSP70,TNF-α and IL-6 （rs = 0. 579,0. 498 and 0. 609, P〈0.05） with statistical significance. Conclusion miR let-7b might involve in the pathology of heat stroke,it provided a potential biomarker for monitoring patients in high temperature and humidity clinically.
出处
《现代检验医学杂志》
CAS
2017年第2期19-22,共4页
Journal of Modern Laboratory Medicine
基金
973计划(2013CB531606)
国家自然科学基金(81471605
81401358
81501397
31500721
81501398
81302579
81273282
81202353)
上海申康基金(SHDC22014014)
上海教育科学基金(D14017)
军队科研基金(BWS14J023
12MA056
15ZD009
15XD007)
美捷登基金(MJR20150019)
