摘要
目的 探讨子宫颈癌放疗患者放疗前后肿瘤组织中Ku70蛋白的表达变化及其与临床病理特征的关系.方法 选择70例接受三维适形放疗联合以铂类为基础化疗的晚期子宫颈癌患者为研究对象,采集放疗前和放疗后不同剂量组（≤10 Gy、≥20 Gy）患者的子宫颈癌组织,应用免疫组织化学技术检测Ku70蛋白的表达,并利用图像分析软件定量分析Ku70蛋白在子宫颈癌组织中的相对表达量.结果Ku70蛋白的相对表达量在≥20 Gy组（1.31±0.83）和≤10 Gy组（0.71±0.60）中均高于放疗前（0.50±0.19）,差异均有统计学意义（t=7.143,P〈0.01;t=2.655,P〈0.05）.Ku70蛋白的表达与临床分期、病理分化程度及肉眼肿瘤直径存在相关性（均P〈0.05）,与患者年龄及肿瘤分型无相关性（均P〉0.05）.放疗前Ku70蛋白在放疗抵抗组中的表达（0.55±0.24）高于放疗敏感组（0.25±0.17）,差异有统计学意义（t=2.979,P〈0.01）.结论 子宫颈癌组织中Ku70蛋白的表达随放疗剂量的增大而升高,且放疗前Ku70蛋白在放疗抵抗组中的表达高于放疗敏感组,提示Ku70蛋白表达与子宫颈癌放疗敏感性有关. Ku70蛋白的表达还与临床分期、病理分化及肉眼肿瘤直径存在相关性.
Objective To investigate expression change of Ku70 protein before and after radiotherapy in patients with cervical cancer, as well as its relationship with clinical characteristics. Methods Seventy cervical cancer patients who received three dimensional conformal radiotherapy combined with chemotherapy were registered. Specimens tissues were obtained from cervical tumors of irradiation dose of ≤10 Gy and ≥20 Gy group. Immunohistochemistry was used to detect the expression of Ku70 in 70 cases, and its relative expression was analyzed by image software. Results The relative expression levels of Ku70 in cervical cancer patients treated with radiation doses of ≥20 Gy group （1.31 ± 0.83） and ≤10 Gy group （0.71 ± 0.60） were significantly higher than that before radiotherapy （0.50 ± 0.19）, and there was a significant difference （t=7.143, P 0.05）. The expression of Ku70 in radiotherapy resistant group was significantly higher than that in radiotherapy sensitive group （0.55 ± 0.24 vs. 0.25 ± 0.17, t= 2.979, P〈 0.01）. Conclusions The expression of Ku70 protein in cervical cancer tissues is increased with the rise of radiotherapy dose. The expression of Ku70 in radiotherapy resistant group is significantly higher than that in radiotherapy sensitive group, indicating that Ku70 protein is correlated with the radiosensitivity, clinical stage, differential degree and tumor diameter.
出处
《肿瘤研究与临床》
CAS
2017年第3期168-171,共4页
Cancer Research and Clinic
关键词
宫颈肿瘤
放射疗法
计算机辅助
免疫组织化学
Ku70蛋白
Uterine cervical neoplasms
Radiotherapy
computer-assisted
Immunohistochemistry
Ku70 protein
