摘要
以大根唇柱苣苔叶片为外植体,以MS为基本培养基,附加不同种类和浓度的激素进行组培快繁技术研究。结果表明:适宜叶片不定芽诱导的培养基是MS+6-BA 1.00 mg/L+NAA 0.05 mg/L,继代增殖培养的适宜培养基为MS+6-BA 1.50~3.00 mg/L+NAA 0.10 mg/L,适宜的生根培养基为1/2MS+IBA 0.20~0.30 mg/L,适宜的基质是泥炭∶珍珠岩=2∶1、泥炭∶细河沙=2∶1、泥炭∶园土=2∶1。
The leaf of Chirita macrorhiza was used as the explants and cultured on MS medium supplemented with different concentrations of plant hormones. The results showed that： the adventitious bud induction culture medium was MS＋6-BA 1.00 mg/L＋NAA 0.05 mg/L. The shoots proliferation medium was MS ＋6-BA 1.50-3.00 mg/L＋NAA0.10 mg/L. The root induction medium was 1/2MS＋IBA 0.20-0.30 mg/L. The suitable ratio of transplanting medium for peat ∶ perlite was 2 ∶ 1, peat ∶ fine sand is 2 ∶ 1, and peat ∶ soil was 2 ∶ 1.
出处
《热带作物学报》
CSCD
北大核心
2017年第3期508-512,共5页
Chinese Journal of Tropical Crops
基金
广西林业科技项目(桂林科字2013第5号)
广西自然科学基金项目(No.2014GXNSFBA118091)
国家林业局部门预算广西项目(桂林护预2015007)
广西壮族自治区林业科学研究院基本科研业务费项目(林科201405号)
关键词
大根唇柱苣苔
组培
快繁
不定芽
Chirita macrorhiza
tissue culture
rapid regeneration
adventitious bud
