摘要
以香石竹(Dianthus caryophyllus)‘马斯特’为试验材料,在分析香石竹和石竹转录组数据的基础上克隆得到10个水孔蛋白(AQP)基因,7个属于PIP亚家族,3个属于TIP亚家族;其中DcaAQP1、DcaAQP2、DcaAQP3、DcaAQP4、DcaAQP6、DcaAQP8和DcaAQP10在萼片中优势表达,DcaAQP5在茎和花瓣中优势表达,DcaAQP7在叶中优势表达,DcaAQP9在各个组织中表达均比较低;在切花开放萎蔫过程中,DcaAQP1、DcaAQP3和DcaAQP6的表达量从花蕾期开始升高,半开期达到最高,DcaAQP4、DcaAQP7、DcaAQP9和DcaAQP10在盛开期表达量达到最高;DcaAQP2在花蕾期表达量最高,DcaAQP5在开始萎蔫期表达量达到最高,表明多个AQP基因协同作用来维持切花开放萎蔫过程中花瓣细胞水分吸收和营养物质转运。
Ten aquaporin genes were cloned from Dianthus caryophyllus ＇Master＇ based on the analysis from D. caryophyllus and D. chinensis transcriptome, 7 genes belonged to PIP subfamily and another 3 belonged to TIP subfamily; Dcad QP1, DcaA QP2 , DcaA QP 3 , DcaA QP 4 , DcaA QP 6 , DcaA QP8 and DcaAQPIO had advantage expression in sepals, and DcaAQP5 had predominant expression in stem and petals; DcaAQP7 had advantage expression in leaves; while the DcaAQP9 expressed low in all tissues; During the flowers opening and wilting process, the expression levels ofDcaAQP1, DcaAQP3 and DcaAQP6 increased from flower bud stage and to the highest at half bloom stage; DcaAQP4, DcaAQPT, DcaAQP9 and DcaAQPIO expressions were the highest at full bloom stage. DcaAQP2 had the highest expression at flower bud stage; DcadQP5 reached the highest level at the beginning of wilting stage. Indicating multiple AQP genes acted synergistically to maintain the water uptake and nutrients transport during the flowers opening and wilting process.
出处
《园艺学报》
CAS
CSCD
北大核心
2017年第3期515-527,共13页
Acta Horticulturae Sinica
基金
国家自然科学基金项目(31000918)
中央高校基本科研业务费专项(2662015PY052
2662016PY041)
关键词
香石竹
水孔蛋白
基因克隆
表达分析
carnation
aquaporin
cloning gene
expression analysis
