摘要
以江津甜橙的低酸小果变异品种‘长叶橙’(Citrus sinensis L.Osbeck‘Changyecheng’)和高酸大果芽变品种‘大果锦橙’(C.sinensis L.Osbeck‘Daguo Jincheng’)为材料,对其AREB(ABA responsive element binding)/ABFs(ABRE binding factors)转录因子基因,即CsAREB1和CsAREB2进行了生物信息学及时空表达分析;采用酶联免疫法(ELISA)测定了不同发育时期果实内源激素(ABA、IAA、GA和ZR)含量。结果表明:CsAREB1的开放阅读框(ORF)长度为1 338 bp,编码445个氨基酸;CsAREB2的ORF为1 347 bp,编码448个氨基酸。CsAREB1和CsAREB2在叶、花、果中的表达存在组织特异性。在果实发育过程中,CsAREB1的表达量在‘长叶橙’中呈上升趋势,在‘大果锦橙’中呈先降后升趋势;CsAREB2的表达量在2个品种中总体均呈上升趋势。在果实细胞分裂期,‘长叶橙’果实CsAREB1、CsAREB2的表达量高于‘大果锦橙’,而在果实膨大期的果皮组织中‘长叶橙’低于‘大果锦橙’,在果肉组织中没有显著差异。两品种CsAREB1和CsAREB2的表达均受外源ABA的诱导。在细胞分裂期和膨大期的果皮组织中‘大果锦橙’中IAA、GA、ZR含量,以及IAA、GA、ZR与ABA的比值均高于‘长叶橙’,而在膨大期果肉组织中这一比值低于‘长叶橙’。随着果实的发育,CsAREB1和CsAREB2的表达量变化与果实内源ABA的含量变化趋势基本一致,而与其他内源激素的含量变化无明显相关关系。研究结果表明:CsAREB1和CsAREB2的转录水平受ABA的调控,通过参与柑橘果实ABA生成,影响柑橘果实体积发育过程。
Using low-acid small fruit variety‘Changyecheng’and high-acid large fruit variety ‘Daguo Jincheng’both mutated from Jiangjin sweet orange as materials,two AREB（ABA responsive element binding）/ABFs（ABRE binding factors）transcription factors,i.e.CsAREB1 and CsAREB2 were analyzed by bioinformatics and expression analysis.In addition,the contents of endogenous hormones including ABA,IAA,GA and ZR in different stages of fruit development were determined with Enzyme Linked Immunosorbent Assay.The results showed that the Open Reading Frames（ORFs）of CsAREB1 and CsAREB2 contained 1 338 bp and 1 347 bp,encoding a protein of 445 and 448 amino acid residues,respectively.CsAREB1 and CsAREB2 predominantly expressed in leaves,flowers and fruits.During the fruit development,the expression level of CsAREB1 showed an increasing pattern in‘Changyecheng’and an increasing firstly and then decreasing pattern in‘Daguo Jincheng’,and the expression of CsAREB2 in both cultivars showed an increasing pattern.These two genes expressed more highly in‘Changyecheng’than in‘Daguo Jincheng’in division stage,conversely in rind and no significant difference in the pulp during expanding stage.The expression of CsAREB1 and CsAREB2 were induced by exogenous ABA.The contents of IAA,GA,ZR and the ratio of IAA,GA,ZR to ABA in‘Daguo Jincheng’were higher than those in‘Changyecheng’in division and expanding stage,however,an opposite trend was observed in flesh tissue in division stage.During fruit development,the expression levels of CsAREB1 and CsAREB2 were positively related to the content of endogenous ABA in fruit,while not to other phytohormones.This study showed that CsAREB1 and CsAREB2 were regulated by ABA,and play a potential role in citrus fruit volume through the regulation of ABA.
出处
《园艺学报》
CAS
CSCD
北大核心
2017年第3期441-451,共11页
Acta Horticulturae Sinica
基金
重庆市研究生科研创新项目(CYS2015071)
重庆市科技支撑示范工程课题(cstc2014fazktjcsf80033)
重庆市自然科学基金项目(cstc2015jcyj A80039)
